玉米大斑病胁迫下球孢白僵菌对玉米植株的影响及定殖规律

doi:10.16409/j.cnki.2095-039x.2022.05.008

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (4): 804-812.DOI: 10.16409/j.cnki.2095-039x.2022.05.008

玉米大斑病胁迫下球孢白僵菌对玉米植株的影响及定殖规律

隋丽¹, 路杨¹, 迟瑞凯^1,2, 赵宇¹, 张正坤¹, 李启云¹

1. 吉林省农业科学院植物保护研究所/吉林省农业微生物重点实验室/农业农村部东北作物有害生物综合治理重点实验室, 公主岭 136100;
2. 吉林农业大学植物保护学院, 长春 130118

收稿日期:2022-04-19 出版日期:2023-08-25 发布日期:2023-08-25
通讯作者: 张正坤, 研究员, E-mail:zhangzhengkun1980@126.com;李启云, 研究员, E-mail:qyli1225@126.com
作者简介:隋丽，研究员，E-mail：suiyaoyi@163.com；路杨，研究员，E-mail：jluluyang@163.com。
基金资助:
吉林省农业科技创新工程重大项目（CXGC2021ZD008）；吉林省农业科学院基本科研经费项目（KYJF2021ZR008）

Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection

SUI Li¹, LU Yang¹, CHI Ruikai^1,2, ZHAO Yu¹, ZHANG Zhengkun¹, LI Qiyun¹

1. Institute of Plant Protection, Jilin Academy of Agricultural Science/Jilin Key Laboratory of Agricultural Microbiology/Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Gongzhuling 136100, China;
2. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China

Received:2022-04-19 Online:2023-08-25 Published:2023-08-25

摘要/Abstract

摘要： 为明确球孢白僵菌在玉米植株中的定殖能力及其对玉米植株生长和抗病性的影响,本研究采用灌根方式构建球孢白僵菌-玉米共生体,对玉米植株叶绿素、氮含量以及株高进行测定,评价球孢白僵菌对玉米的促生作用;通过人工接种玉米大斑病,探究球孢白僵菌定殖对玉米大斑病菌的抗性作用;采用球孢白僵菌菌落计数和荧光定量PCR等方法研究玉米植株受到玉米大斑病菌胁迫时,球孢白僵菌在玉米植株内的定殖扩散规律。结果表明,球孢白僵菌在玉米植株内的定殖率为68.18%,定殖后能够对植株生长产生积极影响,同时能显著降低玉米大斑病的发病率,接种玉米大斑病第21 d发病率降低46.67%。在玉米叶片受到大斑病菌侵染时,能够从根际土壤"招募"球孢白僵菌,使球孢白僵菌在叶片聚集,进而提高对病原微生物的抗性。本研究进一步明确了虫生真菌对植物病害的生防潜力,为虫生真菌在植物组织中定殖研究以及诱导抗性研究提供理论依据。

关键词: 虫生真菌, 内生定殖, 植物病原菌, 招募作用

Abstract: This study aims to clarify the colonization ability of Beauveria bassiana in maize plants and its influence on disease resistance of host plants. B. bassiana-maize symbiosis was constructed by root drenching method and chlorophyll and nitrogen content, as well as plant height were measured to evaluate the growth promoting effect of B. bassiana colonization in maize. The resistance of B. bassiana colonization against Exserohilum turcicum infection was investigated by inoculation of the disease. The colonization and spread of B. bassiana endophytic in maize plants under E. turcicum infection stress was studied by colony counting and quantitative PCR. The results showed that the colonization of B. bassiana in maize reached 68.18%, positively promoted plant growth and significantly reduced the incidence of B. bassiana by 46.67% at 21 day after inoculation. B. bassiana could be 'recruited' from the rhizosphere soil when maize leaves were infected by E. turcicum, and B. bassiana could accumulate at leaves in turn to improve resistance against plant pathogen infection. This study has further clarified the biocontrol potential of entomogenous fungi against plant diseases as endophyte, and provides a theoretical basis for the study of colonization and induced resistance of entomogenous fungi in plant tissues.

Key words: entomopathogenic fungi, endophytic, plant pathogen, recruitment function

中图分类号:

S476.1

隋丽, 路杨, 迟瑞凯, 赵宇, 张正坤, 李启云. 玉米大斑病胁迫下球孢白僵菌对玉米植株的影响及定殖规律[J]. 中国生物防治学报, 2023, 39(4): 804-812.

SUI Li, LU Yang, CHI Ruikai, ZHAO Yu, ZHANG Zhengkun, LI Qiyun. Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection[J]. Chinese Journal of Biological Control, 2023, 39(4): 804-812.

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玉米大斑病胁迫下球孢白僵菌对玉米植株的影响及定殖规律

Effects and Colonization of Beauveria bassiana in Maize under Stress of Exserohilum turcicum Infection

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