四种蒿属植物精油对斑翅果蝇的生物活性

doi:10.16409/j.cnki.2095-039x.2023.01.034

摘要/Abstract

摘要： 斑翅果蝇因其传播速度快，传播范围广的特点对全球水果产业造成了严重危害。本文利用4种蒿属植物精油，即华北米蒿精油、猪毛蒿精油、莳萝蒿精油和黄花蒿精油，对斑翅果蝇开展了生物防治研究，系统探讨了4种精油的杀虫活性。本研究，旨在挖掘新型环保的植物源杀虫剂。利用熏蒸、驱避和对幼虫的致死效应，探究4种精油对斑翅果蝇的生物活性；采用气相色谱-质谱联用仪（GC-MS）对4种蒿属精油的化学成分进行分析。熏蒸试验表明4种精油均具有较好的杀虫特性，其中华北米蒿精油对果蝇的熏蒸效果最好，LC₅₀为1.421 μL/mL，莳萝蒿精油次之，LC₅₀为3.499 μL/mL，黄花蒿精油效果最差；驱避试验表明华北米蒿精油驱虫效果最佳，驱虫率为86%，黄花蒿精油驱虫效果最差，驱虫率为50%；对幼虫的致死效应表明，在猪毛蒿精油作用下，斑翅果蝇幼虫致死率达到70%，莳萝蒿精油效果最差，幼虫死亡率为40%；GC-MS分析结果表明，四种精油共82种成分，并且β-石竹烯、4-萜品醇和1,8-桉叶素是4种精油中的常见化合物。综上研究结果表明，四种精油对斑翅果蝇有较强的杀虫效果，值得进一步研究，并为开发绿色无公害的植物源杀虫剂奠定了理论基础。

关键词: 斑翅果蝇, 蒿属植物精油, 熏蒸活性, 驱避活性, 孵化率, GC-MS

Abstract: Drosophila suzukii Matsumura is a serious threat to fruit production around the world due to its fast spread and wide distribution. Essential oils of four Artemisia plants including A. giraldii, A. scoparia, A. anethoides, and A. annua were evaluated for their biological activities against D. suzukii, aiming to exploit new environmentally friendly plant-derived insecticides. The insecticidal activity of the four essential oils against D. suzukii was determined by fumigation test, repellency test and larval mortality test; the chemical composition of the four essential oils was analyzed using gas chromatography-mass spectrometer (GC-MS). The fumigation test showed that the four essential oils all had good insecticidal activities against D. suzukii, and the fumigation activities of A. giraldii essential oil was the best, with LC₅₀ value of 1.421 μL/mL, followed by A. anethoides essential oil, with LC₅₀ value of 3.499 μL/mL, and A. annua essential oil came at the last. The repellent test showed that A. giraldii essential oil was of the highest repellent activities, with repellency indices of 86%, and the A. annua oil was the worst, with repellency indices of 50%. The larval mortality test showed that A. scoparia essential oil resulted in 70% mortality of D. suzukii larvae, while A. anethoides essential oil was the least effective, with only 40% larval mortality. GC-MS analysis showed that there were 82 components in the four essential oils, and β-Caryophyllene, Terpinen-4-ol, and 1,8-Cineole are common compounds in the four essential oils. The above results indicate that the four Artemisia essential oils have the potential to be developed as an insecticide for D. suzukii. This research paves a theoretical foundation for the development of green pollution-free plant-derived pesticides.

Key words: Drosophila suzukii, essential oils of Artemisia, fumigant activity, repellent activity, larvae eclosion rate, GC-MS

中图分类号:

S476

令利军, 王圆圆, 蒋坤玲, 程文婷, 冯升来, 陆璐, 陈学林. 四种蒿属植物精油对斑翅果蝇的生物活性[J]. 中国生物防治学报, 2023, 39(6): 1341-1350.

LING Lijun, WANG Yuanyuan, JIANG Kunling, CHENG Wenting, FENG Shenglai, LU Lu, CHEN Xuelin. Bioactivity of Four Essential Oils of Artemisia Plants against Drosophila suzukii Matsumura[J]. Chinese Journal of Biological Control, 2023, 39(6): 1341-1350.

参考文献

[1] Abad M J, Bedoya L M, Apaza L, et al. The Artemisia L. genus: a review of bioactive essential oils[J]. Molecules, 2012, 17(3): 2542-2566.
[2] Willcox M. Artemisia species: from traditional medicines to modern antimalarials—and back again[J]. The Journal of Alternative and Complementary Medicine, 2009, 15(2): 101-109.
[3] Yang Y C, Lee H S, Clark J M, et al. Insecticidal activity of plant essential oils against Pediculus humanus capitis (Anoplura: Pediculidae)[J]. Journal of Medical Entomology, 2004, 41(4): 699-704.
[4] Clerck C D, Maso S D, Parisi O, et al. Screening of antifungal and antibacterial activity of 90 commercial essential oils against 10 pathogens of agronomical importance[J]. Foods, 2020, 9(10): 1418.
[5] de Araújo Couto H G S, Blank A F, e Silva A M O, et al. Essential oils of basil chemotypes: major compounds, binary mixtures, and antioxidant activity[J]. Food Chemistry, 2019, 293: 446-454.
[6] Rasheed D M, Serag A, Shakour Z T A, et al. Novel trends and applications of multidimensional chromatography in the analysis of food, cosmetics and medicine bearing essential oils[J]. Talanta, 2021, 223: 121710.
[7] da Silva J A T, Yonekura L, Kaganda J, et al. Important secondary metabolites and essential oils of species within the Anthemideae (Asteraceae)[J]. Journal of Herbs, Spices & Medicinal Plants, 2005, 11(1-2): 1-46.
[8] Dutra Q P, Christ J A, Carrijo T T, et al. Phytocytotoxicity of volatile constituents of essential oils from Sparattanthelium Mart. species (Hernandiaceae)[J]. Scientific Reports, 2020, 10(1): 1-11.
[9] Wani A R, Yadav K, Khursheed A, et al. An updated and comprehensive review of the antiviral potential of essential oils and their chemical constituents with special focus on their mechanism of action against various influenza and coronaviruses[J]. Microbial Pathogenesis, 2021, 152: 104620.
[10] Verdeguer M, Sanchez-Moreiras A M, Araniti F. Phytotoxic effects and mechanism of action of essential oils and terpenoids[J]. Plants (Basel), 2020, 9(11): 1571.
[11] 李都, 牛长缨, 李峰奇, 等. 斑翅果蝇气味结合蛋白OBP56h与小分子化合物的结合特征[J]. 中国农业科学, 2019, 52(15): 2616-2623.
[12] Lee J C, Bruck D J, Dreves A J, et al. In focus: spotted wing drosophila, Drosophila suzukii, across perspectives[J]. Pest Management Science, 2011, 67(11): 1349-1351.
[13] 刘佩旋, 刘成, 徐晓蕊, 等. 一种危险性有害生物——斑翅果蝇研究现状[J]. 中国植保导刊, 2017, 37(5): 5-11.
[14] Yeh D A, Drummond F A, Gómez M I, et al. The economic impacts and management of spotted wing drosophila (Drosophila suzukii): the case of wild blueberries in Maine[J]. Journal of Economic Entomology, 2020, 113(3): 1262-1269.
[15] 郭洁, 张艺馨, 周锐, 等. 几种杀虫剂对斑翅果蝇室内毒力测定[J]. 植物检疫, 2017, 31(1): 51-53.
[16] 尚利娜, 袁海滨, 魏春艳, 等. 猪毛蒿精油对玉米象体内4种解毒酶活力的抑制[J]. 东北师范大学学报(自然科学版), 2010, 42(4): 116-120.
[17] Kim J, Jang M, Shin E, et al. Fumigant and contact toxicity of 22 wooden essential oils and their major components against Drosophila suzukii (Diptera: Drosophilidae)[J]. Pesticide Biochemistry and Physiology, 2016, 133: 35-43.
[18] Deb M, Kumar D. Bioactivity and efficacy of essential oils extracted from Artemisia annua against Tribolium casteneum (Herbst. 1797)(Coleoptera: Tenebrionidae): an eco-friendly approach[J]. Ecotoxicology and Environmental Safety, 2020, 189: 109988.
[19] Peris I, Blázquez M A. Comparative GC-MS analysis of bay leaf (Laurus nobilis L.) essential oils in commercial samples[J]. International Journal of Food Properties, 2015, 18(4): 757-762.
[20] 王慧. 3种蒿属植物杀虫成分提取及制剂配制[D]. 哈尔滨: 东北林业大学, 2012.
[21] 武汗青. 浅谈菊科蒿属植物杀虫作用研究进展[J]. 农业科技通讯, 2015, 521(5): 221-223.
[22] Soonwera M, Wongnet O, Sittichok S. Ovicidal effect of essential oils from Zingiberaceae plants and Eucalytus globulus on eggs of head lice, Pediculus humanus capitis De Geer[J]. Phytomedicine, 2018, 47: 93-104.
[23] Yang Y C, Lee S H, Clark J M, et al. Ovicidal and adulticidal activities of Origanum majorana essential oil constituents against insecticide-susceptible and pyrethroid/malathion-resistant Pediculus humanus capitis (Anoplura: Pediculidae)[J]. Journal of Agricultural and Food Chemistry, 2009, 57(6): 2282-2287.
[24] Oftadeh M, Sendi J J, Ebadollahi A. Toxicity and deleterious effects of Artemisia annua essential oil extracts on mulberry pyralid (Glyphodes pyloalis)[J]. Pesticide Biochemistry and Physiology, 2020, 170: 104702.
[25] Guetat A, Al-Ghamdi F A, Osman A K. The genus Artemisia L. in the northern region of Saudi Arabia: essential oil variability and antibacterial activities[J]. Natural Product Research, 2017, 31(5): 598-603.
[26] Kim M J, Park M H, Jeong M K, et al. Radical scavenging activity and anti-obesity effects in 3T3-L1 preadipocyte differentiation of Ssuk (Artemisia princeps Pamp.) extract[J]. Food Science and Biotechnology, 2010, 19(2): 535-540.
[27] Liang J, Wang W, Zheng Y, et al. Bioactivities and chemical constituents of essential oil extracted from Artemisia anethoides against two stored product insects[J]. Journal of Oleo Science, 2017, 66(1): 71-76.
[28] 江晓波, 王萌, 张丽, 等. 黄花蒿残膏的挥发性成分分析及其抑菌和抗氧化活性研究[J]. 食品工业科技, 2015, 36(14): 103-106.
[29] Pinho A I, Wallau G L, Nunes M E M, et al. Fumigant activity of the Psidium guajava var. pomifera (Myrtaceae) essential oil in Drosophila melanogaster by means of oxidative stress[J]. Oxidative Medicine and Cellular Longevity, 2014, 696785.
[30] Jang M, Kim J, Yoon K A, et al. Biological activity of Myrtaceae plant essential oils and their major components against Drosophila suzukii (Diptera: Drosophilidae)[J]. Pest Management Science, 2017, 73(2): 404-409.