链霉菌HBERC-44441活性成分的分离鉴别及其抗菌活性测定

doi:10.16409/j.cnki.2095-039x.2023.02.027

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (3): 615-623.DOI: 10.16409/j.cnki.2095-039x.2023.02.027

• 生防链霉菌研究论文专栏 • 上一篇下一篇

链霉菌HBERC-44441活性成分的分离鉴别及其抗菌活性测定

万中义¹, 方伟¹, 张志刚¹, 吴兆圆¹, 李泰明², 王开梅¹, 杨自文¹

1. 湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心, 武汉 430064;
2. 华中农业大学植物科学技术学院, 武汉 430070

收稿日期:2022-09-20 出版日期:2023-06-08 发布日期:2023-06-25
通讯作者: 杨自文,博士,研究员,E-mail:ziwen.yang@nberc.com。
作者简介:万中义，博士，研究员，E-mail：wanzhongyi1964@126.com。
基金资助:
湖北省中央引导地方科技发展专项（2019ZYYD061）；湖北省农业科技创新中心项目（2021-620-000-001-027）

Isolation, Identification of Active Compounds Derived from Streptomyces HBERC-44441, and Bioassay of Their Antimicrobial Activity

WAN Zhongyi¹, FANG Wei¹, ZHANG Zhigang¹, WU Zhaoyuan¹, LI Taiming², WANG Kaimei¹, YANG Ziwen¹

1. Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesticide, Hubei Agricultural Science and Technology Innovation Center, Wuhan 430064, China;
2. College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China

Received:2022-09-20 Online:2023-06-08 Published:2023-06-25

摘要/Abstract

摘要： 在筛选微生物源杀菌剂的过程中，从湖北省通山县九宫山采集的土样中分离到一株链霉菌，编号为HBERC-44441。经扫描电镜观察和16S rRNA基因序列比对，菌株被鉴定为利迪链霉菌Streptomyces lydicus。室内生物活性测定结果表明，该菌株发酵提取物对水稻纹枯病菌、小麦赤霉病菌、草莓灰霉病菌等具有强烈抑制活性。经分离制备，获得了其中几个活性组份，经波谱学分析，解析了其中4个化合物的化学结构，分别是吲哚-3-甲酸、pimprinine、另有两个pimprinine二聚体，鉴定为新的化合物。抗菌活性测试表明，吲哚-3-甲酸具有较好抗真菌活性，pimprinine及其二聚体抗真菌活性较弱，首次检测到pimprinine对植物重要病原细菌青枯劳尔氏菌有一定活性，其MIC值可达25 μg/mL。

关键词: 利迪链霉菌, 匹马宁, 吲哚-3-甲酸, 化合物, 抗菌活性

Abstract: Streptomyces sp. HBERC-44441 was isolated from the soil sample collected from Jougongshan Mountain, Tongshan County, Hubei Province. Based on scanning electron microscopic (SEM) observation and 16S rRNA gene sequence analysis, the strain was identified as S. lydicus HBERC-44441.The bioassay results showed that the fermentation extracts of the strain exhibited excellent fungicidal activities against some important crop pathogenic fungi such as Rhizoctonia solani, Fusarium culmorum, Botrytis cinerea and so on. Several active compounds were purified by preparative HPLC, and the chemical structures of four compounds were elucidated by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS), they are indol-3-carboxylic acid, pimprinine, and two novel pimprinine dimers, respectively. The antimicrobial activity of pure compounds showed that pimprinine and its dimer had weak activity against some pathogenic fungi, while indol-3-carboxylic acid showed moderate activity. We found that pimprinine had moderate activity against important crop bacterial wilt for the first time, the MIC value is 25 µg/mL.

Key words: Streptomyces lydicus, pimprinine, indol-3-carboxylic acid, compounds, antimicrobial activity

中图分类号:

S476

万中义, 方伟, 张志刚, 吴兆圆, 李泰明, 王开梅, 杨自文. 链霉菌HBERC-44441活性成分的分离鉴别及其抗菌活性测定[J]. 中国生物防治学报, 2023, 39(3): 615-623.

WAN Zhongyi, FANG Wei, ZHANG Zhigang, WU Zhaoyuan, LI Taiming, WANG Kaimei, YANG Ziwen. Isolation, Identification of Active Compounds Derived from Streptomyces HBERC-44441, and Bioassay of Their Antimicrobial Activity[J]. Chinese Journal of Biological Control, 2023, 39(3): 615-623.

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链霉菌HBERC-44441活性成分的分离鉴别及其抗菌活性测定

Isolation, Identification of Active Compounds Derived from Streptomyces HBERC-44441, and Bioassay of Their Antimicrobial Activity

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