抗生素溶杆菌中PAS-LuxR家族转录因子正向调控吩嗪类抗菌物质myxin的生物合成

doi:10.16409/j.cnki.2095-039x.2024.02.027

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (4): 866-873.DOI: 10.16409/j.cnki.2095-039x.2024.02.027

• 研究论文 • 上一篇

抗生素溶杆菌中PAS-LuxR家族转录因子正向调控吩嗪类抗菌物质myxin的生物合成

赵杨扬^1,2, 陈俊菁^1,2, 徐高歌¹, 承心怡¹, 刘凤权^1,3

1. 江苏省农业科学院植物保护研究所, 南京 210014;
2. 海南大学热带农林学院, 海口 570228;
3. 贵州大学农学院, 贵阳 550025

收稿日期:2023-12-18 发布日期:2024-08-06
作者简介:赵杨扬,博士,副研究员,E-mail:yyzhao2016@163.com;通信作者,博士,研究员,E-mail:fqliu20011@sina.com。
基金资助:
国家自然科学基金（32172492，31701840）

PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus

ZHAO Yangyang^1,2, CHEN Junjing^1,2, XU Gaoge¹, CHENG Xinyi¹, LIU Fengquan^1,3

1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China;
3. College of Agriculture, Guizhou University, Guiyang 550025, China

Received:2023-12-18 Published:2024-08-06

摘要/Abstract

摘要： 溶杆菌Lysobacter是一类具有生防潜力的革兰氏阴性细菌。氮氧化吩嗪myxin是从抗生素溶杆菌Lysobacter antibioticus OH13中分离鉴定到的一种对病原细菌、真菌、卵菌等均具有较强拮抗活性的抗菌物质。我们已对myxin的生物合成机制进行了解析，但其生物合成的调控因子还鲜有报道。本研究在myxin生物合成基因簇上游鉴定到PAS-LuxR家族调控因子编码基因LaPhzR，LaPhzR敲除突变体丧失了产生myxin等吩嗪物质的能力，也丧失了对水稻条斑病菌RS105的拮抗能力，且myxin合成基因与解毒基因LaPhzX均不能表达。这些结果表明LaPhzR正向调控myxin的生物合成，且对myxin的生物合成是必需的。当在野生型菌株中过表达LaPhzR，myxin产量可提高1.6倍，这对myxin的开发应用具有重要意义。

关键词: 溶杆菌, 吩嗪, myxin, 转录调控因子, PAS-LuxR

Abstract: Lysobacter, a genus of Gram-negative bacteria, is emerging as a novel source of biocontrol agents against phytopathogenic microorganisms. Previously, we isolated an N-oxide phenazine myxin from Lysobacter antibioticus OH13, demonstrating potent activity against bacteria, fungi, and oomycetes. Furthermore, we elucidated the biosynthetic pathway of myxin, however, there are few reports concerning its regulatory mechanisms. In this study, we identified a PAS-LuxR family transcriptional regulator encoding gene, LaPhzR, located upstream of the myxin biosynthetic gene cluster. Deletion of LaPhzR resulted in deficiency of myxin production and antagonism against Xanthomonas oryzae pv. oryzicola (Xoc) RS105. Additionally, the LaPhzR mutant exhibited impaired expression of myxin biosynthetic genes and detoxification gene LaPhzX. These findings indicate that LaPhzR positively regulates myxin biosynthesis, a crucial process for myxin production. Overexpression of LaPhzR in the wild type strain led to 1.6-fold increase in myxin production, highlighting its significance in myxin development.

Key words: Lysobacter, phenazine, myxin, transcriptional regulator, PAS-LuxR

中图分类号:

S476

赵杨扬, 陈俊菁, 徐高歌, 承心怡, 刘凤权. 抗生素溶杆菌中PAS-LuxR家族转录因子正向调控吩嗪类抗菌物质myxin的生物合成[J]. 中国生物防治学报, 2024, 40(4): 866-873.

ZHAO Yangyang, CHEN Junjing, XU Gaoge, CHENG Xinyi, LIU Fengquan. PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus[J]. Chinese Journal of Biological Control, 2024, 40(4): 866-873.

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抗生素溶杆菌中PAS-LuxR家族转录因子正向调控吩嗪类抗菌物质myxin的生物合成

PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus

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