沉默几丁质酶3基因抑制桃蚜的存活和生殖

doi:10.16409/j.cnki.2095-039x.2025.05.002

中国生物防治学报 ›› 2025, Vol. 41 ›› Issue (6): 1349-1358.DOI: 10.16409/j.cnki.2095-039x.2025.05.002

• 研究论文 • 上一篇

沉默几丁质酶3基因抑制桃蚜的存活和生殖

苗治国¹, 冯文龙², 刘东阳³, 邓全³, 徐蓬军⁴, 吴圣勇⁵, 余佳敏^1,2, 彭江南⁶, 李茂业¹, 刘苏¹

1. 安徽农业大学植物保护学院/农产品质量与生物安全教育部重点实验室, 合肥 230036;
2. 中国烟草总公司四川省公司, 成都 610041;
3. 四川省烟草公司凉山州公司, 西昌 615000;
4. 中国农业科学院烟草研究所, 青岛 266101;
5. 中国农业科学院植物保护研究所, 北京 100193;
6. 中化现代农业安徽有限公司, 合肥 230041

收稿日期:2024-12-18 发布日期:2025-12-22
通讯作者: 余佳敏, 刘苏
作者简介:苗治国，硕士研究生，E-mail：2056256755@qq.com；冯文龙，农艺师，E-mail：674769170@qq.com。通信作者，余佳敏，高级农艺师，E-mail：scycyujm@163.com；刘苏，副教授，E-mail：suliu@ahau.edu.cn。
基金资助:
安徽省教育厅高校自然科学研究项目（2023AH051053）；中国烟草总公司四川省公司科技项目（SCYC202410，SCYC202519）；烟草行业青年科技托举人才项目（YCQTSC202401）；烟草行业烟草病虫害监测与综合治理重点实验室开放课题（KLTPMIMT2023-09，KLTPMIMT2023-07）

Silencing of Chitinase 3 Gene Inhibits Survival and Reproduction of Myzus persicae

MIAO Zhiguo¹, FENG Wenlong², LIU Dongyang³, DENG Quan³, XU Pengjun⁴, WU Shengyong⁵, YU Jiamin^1,2, PENG Jiangnan⁶, LI Maoye¹, LIU Su¹

1. Key Laboratory of Agri-products Quality and Biosafety, Ministry of Education/School of Plant Protection, Anhui Agricultural University, Hefei 230036, China;
2. Sichuan Branch of China National Tobacco Corporation, Chengdu 610041, China;
3. Liangshan Branch of Sichuan Tobacco Company, Xichang 615000, China;
4. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China;
5. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
6. Sinochem Agriculture Holdings Anhui Co. Ltd., Hefei 230041, China

Received:2024-12-18 Published:2025-12-22

摘要/Abstract

摘要： 桃蚜Myzus persicae是重要的农作物害虫，本研究旨在明确桃蚜几丁质酶3（MperCht3）基因的分子特征以及沉默该基因对桃蚜存活和生殖的影响。从桃蚜转录组中鉴定MperCht3并利用生物信息学软件分析其序列特征，通过实时定量PCR测定MperCht3在桃蚜不同发育阶段及20-羟基蜕皮酮（20E）处理后的转录模式，采用病毒介导的基因沉默（virus-induced gene silencing，VIGS）技术抑制MperCht3的转录并分析桃蚜存活率和产蚜量的变化。结果表明，MperCht3基因包含7个外显子，开放阅读框长度为1422 bp，编码的蛋白含有1个保守的几丁质酶结构域，且与豌豆蚜ApisCht3亲缘关系最近。MperCht3的转录水平在1龄和2龄若虫期较低，从3龄若虫期开始显著上升，在4龄若虫期达到最高。20E处理后24 h，MperCht3表达量显著提高。沉默MperCht3后48、72、96和120 h，桃蚜存活率分别为83.4%、78.02%、70.55%和66.38%，均显著低于对照组的存活率。此外，沉默MperCht3导致37.4%的若蚜无法正常蜕皮，雌成蚜的产蚜量也显著减少。本研究结果表明MperCht3在桃蚜的存活和生殖中具有重要作用，可作为潜在的RNA干扰靶标，用于桃蚜生物防治。

关键词: 桃蚜, 几丁质酶, 序列分析, 转录模式, RNA 干扰

Abstract: The green peach aphid, Myzus persicae, is an important agricultural pest. This study aims to elucidate the molecular characteristics of the chitinase gene MperCht3 in Myzus persicae and to unravel the effects of MperCht3-silencing on aphid survival and reproduction. MperCht3 was identified from the M. persicae transcriptome, and its sequence features were analyzed using bioinformatic software. The transcriptional profile of MperCht3 in M. persicae during various developmental stages and following exposure to 20-hydroxyecdysone (20E) were examined using reverse transcription quantitative PCR. The transcription of MperCht3 was suppressed using virus-induced gene silencing (VIGS) technology and the changes in survival rate and offspring number of M. persicae were investigated. The results indicate that the MperCht3 gene consists of seven exons and contains an open reading frame of 1422 bp. The protein encoded by MperCht3 has a conserved chitinase domain and displays the closest phylogenetic relationship with ApisCht3 from Acyrthosiphon pisum. The transcriptional level of MperCht3 was low in the 1 ^st and the 2^nd instars, but increased obviouslyin the 3^rd instars and reached the peak in the 4^th instars. The transcriptional level of MperCht3 was greatly upregulated at 24 h following treatment with 20E. At 48, 72, 96, and 120 h after silencing of MperCht3, the survival rates of M. persicae were 83.4%, 78.02%, 70.55%, and 66.38%, respectively, which were significantly lower than those of the control. In addition, 37.4% of the MperCht3- silenced nymphs displayed incomplete molting phenotypes, and the fecundity of the MperCht3-silenced female adults was significantly reduced. The findings of this study indicate that MperCht3 plays essential roles in survival and reproduction in M. persicae and could be used as a potential RNA interference target for the biological control of M. persicae.

Key words: Myzus persicae, chitinase, sequence analysis, transcriptional pattern, RNA interference

中图分类号:

S435.72

苗治国, 冯文龙, 刘东阳, 邓全, 徐蓬军, 吴圣勇, 余佳敏, 彭江南, 李茂业, 刘苏. 沉默几丁质酶3基因抑制桃蚜的存活和生殖[J]. 中国生物防治学报, 2025, 41(6): 1349-1358.

MIAO Zhiguo, FENG Wenlong, LIU Dongyang, DENG Quan, XU Pengjun, WU Shengyong, YU Jiamin, PENG Jiangnan, LI Maoye, LIU Su. Silencing of Chitinase 3 Gene Inhibits Survival and Reproduction of Myzus persicae[J]. Chinese Journal of Biological Control, 2025, 41(6): 1349-1358.

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沉默几丁质酶3基因抑制桃蚜的存活和生殖

Silencing of Chitinase 3 Gene Inhibits Survival and Reproduction of Myzus persicae

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