Abstract: It was carried out to select proper substitute hosts for mass rearing Sclerodermus guani (Hymenoptera: Bethylidae), an effective parasitoid for biological control of longhorned beetles in China by present experiment. The result showed that male pupae of Apis mellifera ligusteca Spinola (Hymenoptera: Apidae), was successful and that the adult wasps reared with it had high parasitic ability and vigor, and 32 individuals of the wasp adults could be on average reared out by one individual of the substitute host. The lifespan of the female wasps reared was about 29 d, the male 5.5 d at 26℃ respectively, and more than 65.45% female parasitoids could live over a mouth. Five to eight generations of the parasitoid could be reared out in a year with the substitute host. The comparison indicated that the parasitic wasp reared by the substitute host was about the same in body size, viability and parasitic ability with ones reared by the larvae of its original host, Saperda populnea L. (Coleoptera: Cerambycidae). Meanwhile, the cost of the mass rearing by using the substitute was 7.44% of that by using larvae of S. populnea. Thus, it is recommended that the male pupa of Italian honey bee could be a good substitute host for mass rearing the parasitoid to meet the need of the biological control of many longhorned beetles.