Abstract: Artificial medium for rearing Trichogramma dendrolimi Matsumurain vitro was optimized by reducing the content of pupal hemolymph of Antheraea pernyi (Guérin-Méneville), and adding different ratio of Ganoderma lucidum polysaccharides and chitosan oligosaccharide solution. Compared to CK, when hemolymph was reduced from 40% to 30% and Ganoderma lucidum polysaccharides and chitosan oligosaccharide solution was increased by ratio of 10:0, 4:1, 3:2, 1:1, 2:3, 1:4 and 0:10, these new artificial media significantly improved developmental parameters. T. dendrolimi female wasps did not laid eggs in media supplemented with Ganoderma lucidum polysaccharides and chitosan oligosaccharide solution at ratio of 3:2, 2:3 and 1:4, but there were no significant differences in developmental parameters of the wasp, including rates of parasitism, pupation, adult emergence, and normal adults compared to the media with Ganoderma lucidum polysaccharides and chitosan oligosaccharide solution at ratio of 15:0,4:1. T. dendrolimi developed well on the new media with 30% hemolymph than on the media with 25% hemolymph, except on medium with Ganoderma lucidum polysaccharides and chitosan oligosaccharide solution at ratio of 4:1. The results showed that insect hemolymph was a key component of the artificial media. Two artificial media A4 (30% hemolymph, 30% egg yolk, 20% fresh milk, 10% N's salt solution, 5% Ganoderma lucidum polysaccharides solution, 5% chitosan oligosaccharide solution) and B2 (25% hemolymph,30% egg yolk,20% fresh milk,10% N's salt solution,12% Ganoderma lucidum polysaccharides solution,3% chitosan oligosaccharide solution) were screened out, and media A4 had the potential to be used in mass production of T. dendrolimi.