取食四种寄主植物对番茄潜叶蛾中肠肠道细菌的影响

doi:10.16409/j.cnki.2095-039x.2024.02.044

摘要/Abstract

摘要： 番茄潜叶蛾Tuta absoluta是一种世界性害虫，寄主范围广泛，对多种寄主作物造成严重危害。通过16S rRNA测序分析了取食番茄、马铃薯、茄子、和人参果的番茄潜叶蛾2龄幼虫中肠肠道细菌的多样性和功能。结果显示，在上述四种寄主植物为食的番茄潜叶蛾中肠肠道细菌中，共检测到4886、6016、2781、3471个操作分类单元（OTU），其中共有905个OUT。Alpha和Beta多样性分析结果表明，取食马铃薯和茄子的番茄潜叶蛾中肠肠道细菌多样性最高。物种分类分析表明，取食不同寄主的番茄潜叶蛾，中肠肠道细菌在门水平的优势菌群均为厚壁菌门和变形菌门；在属水平上，取食番茄和马铃薯的番茄潜叶蛾中肠肠道细菌优势属为沃尔巴克氏体，取食人参果和茄子的为肠球菌属；对其代谢通路分析，发现取食四种寄主植物其中肠肠道细菌代谢通路相似，无显著差异，且占比最高的代谢通路都为生物合成和降解代谢。

关键词: 番茄潜叶蛾, 不同寄主植物, 中肠肠道细菌, 菌群多样性

Abstract: Tomato leaf miner (Tuta absoluta) is a worldwide pest with a wide range of host species, causing serious harm to its host crops. The mid-gut bacterial diversity and function of its larvae feeding on tomato, potato, eggplant and ginseng, were analyzed by 16S rRNA sequencing. The results showed that 4886, 6016, 2781, and 3471 operational taxonomic units (OTU, respectively) were detected, among which 905 OTUs were common. Alpha and Beta diversity analyses showed mid gut bacterial diversities were the highest in the larvae feeding on potato and eggplant. Species classification analysis showed that, for each host plant, and at each level of classification, mid-gut bacterial species abundance differed. Firmicutes and Proteobacteria were the dominant mid-gut bacterial phyla, Wolbachia was the dominant genus on tomato and potato hosts, and Enterococcus was the dominant genus on ginseng fruit and eggplant hosts. Analyses showed that their metabolic pathways were statistically similar, with the greatest proportion of metabolic pathways for biosynthesis and degradation. In conclusion, though host plant species significantly affect the mid-gut bacterial diversity and community structure of tomato leaf miner larvae, the microbial functions are similar.

Key words: Tuta absoluta, different host plant, midgut bacterium, bacterial diversity

中图分类号:

S476

杨亚贤, 周昭旭, 钱秀娟, 刘月英, 张美娇, 蒋明君. 取食四种寄主植物对番茄潜叶蛾中肠肠道细菌的影响[J]. 中国生物防治学报, 2024, 40(4): 776-786.

YANG Yaxian, ZHOU Zhaoxu, QIAN Xiujuan, LIU Yueying, ZHANG Meijiao, JIANG Mingjun. Effects of Feeding Four Host Plants on Midgut Bacteria of Tuta absoluta[J]. Chinese Journal of Biological Control, 2024, 40(4): 776-786.

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