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中国生物防治学报 ›› 2025, Vol. 41 ›› Issue (6): 1293-1299.DOI: 10.16409/j.cnki.2095-039x.2025.01.020

• 研究论文 •    

不同储存方法对松毛虫赤眼蜂繁殖能力的影响

李蕙1, 张茜瑜2, 郑美鑫1, 宋潇洋1, 郭嘉庆1, 张雪1, 张俊杰1, 史宏伟3   

  1. 1. 吉林省生物防治技术工程研究中心/吉林省农业害虫生物防治国际科技合作重点实验室/吉林农业大学农业现代化综合技术研究所, 长春 130118;
    2. 通化师范学院生命科学学院, 通化 134002;
    3. 吉林省农业技术推广总站, 长春 130033
  • 收稿日期:2024-12-18 发布日期:2025-12-22
  • 通讯作者: 张雪, 史宏伟
  • 作者简介:李蕙,硕士研究生,E-mail:lihuilh1214@163.com。通信作者,张雪,博士,讲师,E-mail:zhangxue@jlau.edu.cn;史宏伟,硕士,研究员,E-mail:625705966@qq.com。
  • 基金资助:
    国家自然科学基金(32102291)

Effects of Different Storage Methods on the Reproductive Potential of Trichogramma dendrolimi

LI Hui1, ZHANG Qianyu2, ZHENG Meixin1, SONG Xiaoyang1, GUO Jiaqing1, ZHANG Xue1, ZHANG Junjie1, SHI Hongwei3   

  1. 1. Jilin Province Technology Research Center of Biological Control Engineering/Jilin Province International Cooperation Key Laboratory for Biological Control of Agricultural Pests/Institute of Agricultural Modernization Comprehensive Technology, Jilin Agricultural University, Changchun 130118, China;
    2. School of Life Sciences, Tonghua Normal University, Tonghua 134002, China;
    3. Jilin Agricultural Technology Extension Station, Changchun 130033, China
  • Received:2024-12-18 Published:2025-12-22

摘要: 为明确诱导滞育和冷藏两种储存方法对松毛虫赤眼蜂成蜂繁殖能力的影响,本研究分别通过滞育30 d和低温冷藏30 d处理松毛虫赤眼蜂,同时以常温发育(26℃)的松毛虫赤眼蜂为对照,统计各组雌蜂繁殖力(终生产卵量)、首日产卵占比、怀卵量、卵巢内成熟卵子占比、寿命、产卵天数和产卵持续时间等生殖行为指标。结果表明,与常温发育相比,滞育和冷藏显著提高了松毛虫赤眼蜂的怀卵量和终生产卵量,并降低了首日产卵占比。不同之处在于,滞育显著提升了松毛虫赤眼蜂的成熟卵子占比及其寿命,而冷藏则导致这两项指标的下降。此外,滞育处理的松毛虫赤眼蜂显著缩短了产卵行为所需时间,而冷藏处理则使得所需时间增加。然而,在产卵天数方面,三种处理之间并未观察到显著差异。滞育与冷藏相比,经历滞育的松毛虫赤眼蜂在本研究调查的各项指标上均优于冷藏个体。在贮存天数为30 d时,滞育明显提升了松毛虫赤眼蜂的整体质量,而冷藏对其质量产生了一定程度的负面影响。

关键词: 滞育, 冷藏, 产卵量, 产卵行为

Abstract: This study aimed to investigate the differences in reproductive potential of Trichogramma dendrolimi after diapause induction and cold storage. The findings are expected to provide references for the mass-rearing, storage, and application of T. dendrolimi. In this research, the Trichogramma insects were either maintained a 30-day diapause or kept in a 30-day cold storage. Seven biological indicators were measured: the number of parasitized host eggs, the proportion of wasp eggs laid on the first day, the number of wasp eggs in ovary, the proportion of mature wasp eggs, longevity, oviposition duration, and duration of laying behavior. The results showed that, compared with the wasps maintained at normal temperature, diapause and cold storage significantly increased the number of eggs in ovary and lifetime eggs, and decreased the proportion of eggs laid on the first day. Diapause significantly increased the proportion of mature eggs and longevity in T. dendrolimi, while refrigeration led to a decline in both indicators. In addition, diapause treatment significantly shortened the time required for laying behavior, while cold storage increased the time required. However, no significant differences in oviposition duration were observed between the three treatments. Compared with cold storage wasps, diapause T. dendrolimi were superior in all the indexes investigated in this study. When the storage duration was 30 days, diapause obviously improved the overall quality of T. dendrolimi, while cold storage had a certain degree of negative impact.

Key words: diapause, cold storage, fecundity, oviposition behavior

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