丽蚜小蜂糖转运蛋白基因克隆及其诱导表达

doi:10.16409/j.cnki.2095-039x.2018.02.011

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (2): 247-253.DOI: 10.16409/j.cnki.2095-039x.2018.02.011

丽蚜小蜂糖转运蛋白基因克隆及其诱导表达

刘贻聪, 张友军, 吴青君, 谢文, 王少丽

中国农业科学院蔬菜花卉研究所, 北京 100081

收稿日期:2017-11-20 出版日期:2018-04-08 发布日期:2018-04-13
通讯作者: 王少丽,研究员,E-mail:wangshaoli@caas.cn。
作者简介:刘贻聪,硕士研究生,E-mail:yicongliu0829@163.com
基金资助:
国家重点研发计划（2017YFD0200400）；国家现代化农业产业技术体系（CARS-25）；国家自然科学基金（31572014）；农业部园艺作物生物学与种质创制重点实验室基金

Cloning and Induced Expression of Sugar Transporter Gene in Parasitoid, Encarsia formosa Gahan

LIU Yicong, ZHANG Youjun, WU Qingjun, XIE Wen, WANG Shaoli

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2017-11-20 Online:2018-04-08 Published:2018-04-13

摘要/Abstract

摘要： 丽蚜小蜂Encarsia formosa Gahan是粉虱类害虫的重要寄生性天敌昆虫，补充糖分等营养可以延长丽蚜小蜂寿命及增加其产卵量，但对其糖转运相关蛋白基因及其糖诱导表达尚不清楚。本研究克隆获得丽蚜小蜂的糖转运蛋白EfST1基因，与其他寄生蜂的糖转运蛋白基因序列同源性达65%以上；进一步采用实时荧光定量qPCR技术检测发现，饲喂不同浓度的葡萄糖溶液均可显著提高EfST1基因的表达量，且饲喂不同时间段（2～48 h）后，丽蚜小蜂EfST1基因表达量均显著高于对照组，2 h和48 h处理组间差异不显著。综上，EfST1基因可能与糖分补充及其转运密切相关，该基因诱导表达受糖分浓度影响小。研究结果为进一步研究寄生蜂生物防治过程中补充营养及其转运机制奠定基础。

关键词: 丽蚜小蜂, 烟粉虱, CODEHOP, 糖转运蛋白基因, 表达分析

Abstract: Encarsia formosa Gahan is a kind of endoparasitoid world widely used for control of pest whiteflies. Sugar supplement prolong longevity and/or increase their egg production; however, the sugar transporter gene and the induced expression in E. formosa was unclear. In this present study, the sugar transporter gene named EfST1 was cloned; sequence alignment and phylogenetic analysis revealed that EfST1 shared more than 65% homology with other parasitoids. Real-time PCR showed that EfST1 was significantly expressed by feeding different concentrations of glucose. After feeding sugar for different periods (2-48 h) on 10% glucose, EfST1 gene expression was found to be increased compared to the water control. Besides, there were no significant differences between the 2 h and 48 h feeding treatments. In conclusion, the EfST1 gene was probably related to sugar supplement and transportation; and the gene expression induced by different sugar concentrations was not greatly fluctuated. The results will provide a basis for studying the nutrition supplement and transportation mechanism during the biological control of parasitoids.

Key words: Encarsia formosa, Bemisa tabaci, CODEHOP, sugar transporter gene, expression analysis

中图分类号:

S476.3

刘贻聪, 张友军, 吴青君, 谢文, 王少丽. 丽蚜小蜂糖转运蛋白基因克隆及其诱导表达[J]. 中国生物防治学报, 2018, 34(2): 247-253.

LIU Yicong, ZHANG Youjun, WU Qingjun, XIE Wen, WANG Shaoli. Cloning and Induced Expression of Sugar Transporter Gene in Parasitoid, Encarsia formosa Gahan[J]. journal1, 2018, 34(2): 247-253.

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丽蚜小蜂糖转运蛋白基因克隆及其诱导表达

Cloning and Induced Expression of Sugar Transporter Gene in Parasitoid, Encarsia formosa Gahan

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