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中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (6): 876-883.DOI: 10.16409/j.cnki.2095-039x.2019.06.013

• 研究论文 • 上一篇    下一篇

玫烟色棒束孢FZ-01鉴定及对烟粉虱的防治潜力评估

郑宇1,2, 丁雪玲1,2, 姚凤銮1,2, 卢学松1,2, 卓辉3, 何玉仙1,2   

  1. 1. 福建省农业科学院植物保护研究所/农业部福州作物有害生物科学观测实验站/福建省作物有害生物监测与治理重点实验室, 福州 350013;
    2. 闽台作物有害生物生态防控国家重点实验室/福建农林大学, 福州 350002;
    3. 福建中医药大学药学院, 福州 350108
  • 收稿日期:2019-02-26 出版日期:2019-12-08 发布日期:2019-12-18
  • 通讯作者: 何玉仙,博士,研究员,E-mail:yuxianhe_faas@sina.cn
  • 作者简介:郑宇,博士,副研究员,E-mail:290896369@qq.com。
  • 基金资助:
    福建省公益类科研院所专项(2015R1024-4,2017R1025-1);福建省科技重大专项(2017NZ0003-1)

Identification of Isaria fumosorosea FZ-01 and Evaluation of Its Bio-control Potential against Bemisia tabaci

ZHENG Yu1,2, DING Xueling1,2, YAO Fengluan1,2, LU Xuesong1,2, ZUO Hui3, HE Yuxian1,2   

  1. 1. Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
    2. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    3. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
  • Received:2019-02-26 Online:2019-12-08 Published:2019-12-18

摘要: 为了明确从罹病烟粉虱Bemisia tabaci上分离的虫生真菌(菌株FZ-01)的分类归属及其生物防治的潜力,采用形态学特征观察和rDNA-ITS序列分析方法对该菌株进行鉴定,并对其生物防治潜力进行评估。结果发现:菌株FZ-01为玫烟色棒束孢Isaria fumosorosea;该菌株在马铃薯PDA培养基上生长良好;适应温度和酸碱度范围较广,可耐高温(35℃)和酸碱(pH4~10);孢子萌发速率快(5 h)且萌发率高(>90%);菌株致病力测定表明分生孢子浓度为1.0×107 cfu/mL时,对烟粉虱(MEAM1)成虫致死中时间(LT50)仅为2.75 d,接菌4 d时致死中浓度(LC50)仅为2.69×104 cfu/mL。综上结果表明,玫烟色棒束孢FZ-01对烟粉虱具有十分优良的生防潜力。

关键词: 虫生真菌, 玫烟色棒束孢, 烟粉虱, 生防潜力

Abstract: A strain of entomopathogenic fungi (FZ-01) isolated from Bemisia tabaci was identified morphologically and using molecular method employing rDNA-ITS gene sequences. The biocontrol potential of the strain was also evaluated. The strain FZ-01 was identified as Isaria fumosorosea, growing well on the potato medium (PDA), showing wide adaptability to temperatures (above 35℃) and pH values (pH 4-10), and with fast spore germination speed (5 h) and high spore germination rate (>90%). Under laboratory conditions, the virulence test showed that the strain at conidium concentration of 1.0×107cfu/mL was characterized by the medium lethal time (LT50) of 2.75 d and the medium lethal concentration (LC50) of 2.69×104cfu/mL at 4 d after inoculation. The results indicate that the strain FZ-01 possesses promising biocontrol potential against B. tabaci.

Key words: entomopathogenic fungi, Isaria fumosorosea, Bemisia tabaci, bio-control potential

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