中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (2): 317-327.DOI: 10.16409/j.cnki.2095-039x.2026.02.032
• 创刊40周年纪念专栏 • 上一篇
刘紫依, 王震铄, 李燕, 曾庆超, 王琦
收稿日期:2026-03-18
发布日期:2026-05-14
通讯作者:
王琦,博士,教授,E-mail:wangqi@cau.edu.cn。
作者简介:刘紫依,博士研究生,E-mail:liuziyi201620@163.com;王震铄,博士,副教授,E-mail:zhenswang@163.com;
基金资助:LIU Ziyi, WANG Zhenshuo, LI Yan, ZENG Qingchao, WANG Qi
Received:2026-03-18
Published:2026-05-14
摘要: 植物病害严重威胁全球农作物的产量与品质,长期依赖化学农药防治易引发病原菌抗药性增强、农药残留和环境污染等问题,因此环境友好型的生物防治逐渐受到广泛关注。芽胞杆菌Bacillus spp.是重要的植物有益微生物,在植物病害生物防治中具有广阔应用前景。近年来,随着分子生物学和多组学技术的发展,芽胞杆菌的资源挖掘和作用机制研究不断深入,其生防菌剂的开发与应用也取得了重要进展。本文系统综述了生防芽胞杆菌的研究进展,包括资源挖掘、作用机制与产品开发应用,并对其未来研究重点及应用发展趋势进行了展望。
中图分类号:
刘紫依, 王震铄, 李燕, 曾庆超, 王琦. 植物病害生防芽胞杆菌研究进展[J]. 中国生物防治学报, 2026, 42(2): 317-327.
LIU Ziyi, WANG Zhenshuo, LI Yan, ZENG Qingchao, WANG Qi. Research Progress on Biocontrol Bacillus spp. for Plant Disease Management[J]. Chinese Journal of Biological Control, 2026, 42(2): 317-327.
| [1] Godfray H C J, Beddington J R, Crute I R, et al. Food security: the challenge of feeding 9 billion people[J]. Science, 2010, 327(5967): 812-818. [2] Zhang N, Wang Z, Shao J, et al. Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture[J]. Microbial Biotechnology, 2023, 16(12): 2250-2263. [3] Lahlali R, Ezrari S, Radouane N, et al. Biological control of plant pathogens: a global perspective[J]. Microorganisms, 2022, 10(3): 596. [4] 彭钿钿, 马云龙, 许沛冬, 等. 芽胞杆菌防治植物病害作用机制与应用[J]. 生物技术通报, 2025, 8(41): 42-52. [5] Earl A M, Losick R, Kolter R. Ecology and genomics of Bacillus subtilis[J]. Trends in Microbiology, 2008, 16(6): 269-275. [6] Akinsemolu A A, Onyeaka H, Odion S, et al. Exploring Bacillus subtilis: Ecology, biotechnological applications, and future prospects[J]. Journal of Basic Microbiology, 2024, 64(6): 2300614. [7] Nicholson W L, Munakata N, Horneck G, et al. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments[J]. Microbiology and Molecular Biology Reviews, 2000, 64(3): 548-572. [8] 王阶平, 刘波, 刘国红, 等. 芽胞杆菌系统分类研究最新进展[J]. 福建农业学报, 2017, 32(7): 784-800. [9] Li P, Tedersoo L, Crowther TW, et al. Fossil-fuel-dependent scenarios could lead to a significant decline of global plant-beneficial bacteria abundance in soils by 2100[J]. Nature Food, 2023, 4(11): 996-1006. [10] Patel S, Gupta R S. A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 2020, 70(1): 406-438. [11] Zhao M, Liu D, Liang Z, et al. Antagonistic activity of Bacillus subtilis CW14 and its β-glucanase against Aspergillus ochraceus[J]. Food Control, 2022, 131: 108475. [12] Deng Y, Chen Z, Ruan C, et al. Antifungal activities of Bacillus velezensis FJAT 52631 and its lipopeptides against anthracnose pathogen ‐ Colletotrichum acutatum[J]. Journal of Basic Microbiology, 2023, 63(6): 594-603. [13] Tran TD, Del Cid C, Hnasko R, et al. Bacillus amyloliquefaciens ALB65 inhibits the growth of Listeria monocytogenes on cantaloupe melons[J]. Applied and Environmental Microbiology, 2020, 87(1): e01926-20. [14] 邴辉, 王仲康, 杜秉海, 等. 基因组关联分析地衣芽孢杆菌LCDD6对核桃苗的促生作用[J]. 应用与环境生物学报, 2022, 28(3): 588-595. [15] Hao K, Ullah H, Qin X, et al. Effectiveness of Bacillus pumilus PDSLzg-1, an innovative hydrocarbon-degrading bacterium conferring antifungal and plant growth-promoting function[J]. 3 Biotech, 2019, 9(8): 305. [16] Ajdig M, Mbarki A, Chouati T, et al. Comprehensive genomic and pan-genomic analysis of the drought-tolerant Bacillus halotolerans strain OM-41 isolated from olive rhizosphere, reveals potential plant growth-promoting and biocontrol traits[J]. World Journal of Microbiology and Biotechnology, 2025, 41(8): 276. [17] Sorokan A, Gabdrakhmanova V, Kuramshina Z, et al. Plant-associated Bacillus thuringiensis and Bacillus cereus: inside agents for biocontrol and genetic recombination in phytomicrobiome[J]. Plants, 2023, 12(23): 4037. [18] Sharga B M, Lyon G D. Bacillus subtilis BS 107 as an antagonist of potato blackleg and soft rot bacteria[J]. Canadian Journal of Microbiology, 1998, 44(8): 777-783. [19] Blake C, Christensen M N, Kovács Á T. Molecular aspects of plant growth promotion and protection by Bacillus subtilis[J]. Molecular Plant-Microbe Interactions®, 2021, 34(1): 15-25. [20] Santoyo G, Urtis-Flores C A, Loeza-Lara P D, et al. Rhizosphere colonization determinants by plant growth-promoting rhizobacteria (PGPR)[J]. Biology-Basel, 2021, 10(6): 475. [21] Grady E N, MacDonald J, Liu L, et al. Current knowledge and perspectives of Paenibacillus: A review[J]. Microbial Cell Factories, 2016, 15(1): 203. [22] 刘悦, 曾凡松, 龚双军, 等. 解淀粉芽胞杆菌EA19菌株对小麦赤霉病的防治效果[J]. 植物保护学报, 2020, 47(6): 1270-1276. [23] 蒲欣, 吴茂华, 刘锋, 等. 芽孢杆菌对玉米真菌病害生物防治效果的研究进展[J]. 江苏农业科学, 2024, 52(4): 23-30. [24] 乔俊卿, 陈志谊, 梁雪杰, 等. 枯草芽孢杆菌Bs916防治番茄青枯病[J]. 中国生物防治学报, 2016, 32(2): 229-234. [25] 张晓云, 王雪美, 丛蓉, 等. 防治葡萄灰霉病的贝莱斯芽胞杆菌HMB28023筛选及其抑菌物质[J]. 中国生物防治学报, 2025, 41(1): 122-131. [26] Raymaekers K, Ponet L, Holtappels D, et al. Screening for novel biocontrol agents applicable in plant disease management – A review[J]. Biological Control, 2020, 144: 104240. [27] Pliego C, Ramos C, de Vicente A, et al. Screening for candidate bacterial biocontrol agents against soilborne fungal plant pathogens[J]. Plant and Soil, 2011, 340(1): 505-520. [28] 康兴娇, 申红妙, 贾招闪, 等. 葡萄霜霉病生防菌甲基营养型芽胞杆菌T3的鉴定及其防治效果[J]. 中国生物防治学报, 2016, 32: 775-782. [29] 孙旺旺, 闫丽, 陈昌龙, 等. 生菜软腐和菌核病拮抗菌贝莱斯芽胞杆菌BPC6鉴定与防效[J]. 中国生物防治学报, 2020, 36(2): 231-240. [30] 陈川雁, 王燕, 喻国辉, 等. 枯草芽胞杆菌R31影响巴西蕉根系活性氧产生及对枯萎病的防治效果[J]. 中国生物防治学报, 2017, 33(2): 226-233. [31] 何朋杰, 崔文艳, 何鹏飞, 等. 叶面喷施枯草芽胞杆菌XF-1防治大白菜根肿病[J]. 植物保护, 2019, 45(1): 104-108. [32] 李新宇, 李磊, 石延霞, 等. 黄瓜棒孢叶斑病拮抗细菌的筛选、鉴定及防治效果[J]. 植物保护学报, 2020, 47(3): 620-627. [33] 孟焕文, 赵远征, 梁东超, 等. 马铃薯黑胫病生防菌株的筛选与鉴定[J]. 中国植保导刊, 2020, 40(8): 24-29. [34] Ayaz M, Li C-H, Ali Q, et al. Bacterial and fungal biocontrol agents for plant disease protection: journey from lab to field, current status, challenges, and global perspectives[J]. Molecules, 2023, 28(18): 6735. [35] 黎永坚, 程萍, 喻国辉, 等. 枯草芽孢杆菌R31和 TR21菌株防治香蕉枯萎病田间药效试验[J]. 广东农业科学, 2012, 39(23): 70-72. [36] 王鹏程, 金光辉, 张春雨, 等. 不同生防菌剂组合及施用方式对马铃薯疮痂病的防治效果及促生作用[J]. 西南农业学报, 2022, 35(4): 797-803. [37] Elnahal A S M, El-Saadony M T, Saad A M, et al. The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review[J]. European Journal of Plant Pathology, 2022, 162(4): 759-792. [38] Valenzuela Ruiz V, Cervantes Enriquez E P, Vázquez Ramírez M F, et al. A new era in the discovery of biological control bacteria: omics-driven bioprospecting[J]. Soil Systems, 2025, 9(4): 108. [39] Xia L, Miao Y, Cao A, et al. Biosynthetic gene cluster profiling predicts the positive association between antagonism and phylogeny in Bacillus[J]. Nature Communications, 2022, 13(1): 1023. [40] Massart S, Martinez-Medina M, Jijakli M H. Biological control in the microbiome era: challenges and opportunities[J]. Biological Control, 2015, 89: 98-108. [41] Muñoz C Y, Zhou L, Yi Y, et al. Biocontrol properties from phyllospheric bacteria isolated from Solanum lycopersicum and Lactuca sativa and genome mining of antimicrobial gene clusters[J]. BMC Genomics, 2022, 23(1): 152. [42] Gao M, Xiong C, Gao C, et al. Disease-induced changes in plant microbiome assembly and functional adaptation[J]. Microbiome, 2021, 9(1): 187. [43] Wang R, Zhang W, He Z, et al. Core microbiota recruited by healthy grapevines enhance resistance against root rot disease[J]. Genome Biology, 2026, 27(1): 13. [44] Biggs M B, Craig K, Gachango E, et al. Genomics-and machine learning-accelerated discovery of biocontrol bacteria[J]. Phytobiomes Journal, 2021, 5(4): 452-463. [45] Fira D, Dimkić I, Berić T, et al. Biological control of plant pathogens by Bacillus species[J]. Journal of Biotechnology, 2018, 285: 44-55. [46] Calvo H, Mendiara I, Arias E, et al. Antifungal activity of the volatile organic compounds produced by Bacillus velezensis strains against postharvest fungal pathogens[J]. Postharvest Biology and Technology, 2020, 166: 111208. [47] Abriouel H, Franz C M A P, Ben Omar N, et al. Diversity and applications of Bacillus bacteriocins[J]. FEMS Microbiology Reviews, 2011, 35(1): 201-232. [48] Dimkić I, Janakiev T, Petrović M, et al. Plant-associated Bacillus and Pseudomonas antimicrobial activities in plant disease suppression via biological control mechanisms - A review[J]. Physiological and Molecular Plant Pathology, 2022, 117: 101754. [49] Scholz R, Vater J, Budiharjo A, et al. Amylocyclicin, a novel circular bacteriocin produced by Bacillus amyloliquefaciens FZB42[J]. Journal of Bacteriology, 2014, 196(10): 1842-1852. [50] Shelburne CE, An FY, Dholpe V, et al. The spectrum of antimicrobial activity of the bacteriocin subtilosin A[J]. Journal of Antimicrobial Chemotherapy, 2007, 59(2): 297-300. [51] Ongena M, Jacques P. Bacillus lipopeptides: versatile weapons for plant disease biocontrol[J]. Trends in Microbiology, 2008, 16(3): 115-125. [52] 马佳, 李颖, 胡栋, 等. 芽胞杆菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2018, 34(4): 639-648. [53] Bais H P, Fall R, Vivanco J M. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production[J]. Plant Physiology, 2004, 134(1): 307-319. [54] Romero D, de Vicente A, Rakotoaly R H, et al. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca[J]. Molecular Plant-Microbe Interactions, 2007, 20(4): 430-440. [55] Sui X, Han X, Cao J, et al. Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt[J]. Frontiers in Microbiology, 2022, 13: 940156. [56] Zhang L, Sun C. Fengycins, Cyclic lipopeptides from marine Bacillus subtilis strains, kill the plant-pathogenic fungus Magnaporthe grisea by inducing reactive oxygen species production and chromatin condensation[J]. Applied and Environmental Microbiology, 2018, 84(18): e00445-18. [57] Wu L, Wu H, Chen L, et al. Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens[J]. Scientific Reports, 2015, 5(1): 12975. [58] Milner J, Raffel S, Lethbridge B, et al. Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85[J]. Applied Microbiology and Biotechnology, 1995, 43(4): 685-691. [59] Zhang D, Yu S, Yang Y, et al. Antifungal effects of volatiles produced by Bacillus subtilis against Alternaria solani in potato[J]. Frontiers in Microbiology, 2020, 11: 1196. [60] Kai M, Haustein M, Molina F, et al. Bacterial volatiles and their action potential[J]. Applied Microbiology and Biotechnology, 2009, 81(6): 1001-1012. [61] Xu W, Yang Q, Yang F, et al. Evaluation and genome analysis of Bacillus subtilis YB-04 as a potential biocontrol agent against Fusarium wilt and growth promotion agent of cucumber[J]. Frontiers in Microbiology, 2022, 13: 885430. [62] Luo L, Zhao C, Wang E, et al. Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms[J]. Microbiological Research, 2022, 259: 127016. [63] Arnaouteli S, Bamford N C, Stanley-Wall N R, et al. Bacillus subtilis biofilm formation and social interactions[J]. Nature Reviews Microbiology, 2021, 19(9): 600-614. [64] Zhang Y, Gao X, Wang S, et al. Application of Bacillus velezensis NJAU-Z9 enhanced plant growth associated with efficient rhizospheric colonization monitored by qPCR with primers designed from the whole genome sequence[J]. Current Microbiology, 2018, 75(12): 1574-1583. [65] Li S, Zhang N, Zhang Z, et al. Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation[J]. Biology and Fertility of Soils, 2013, 49(3): 295-303. [66] Dimopoulou A, Theologidis I, Benaki D, et al. Direct antibiotic activity of bacillibactin broadens the biocontrol range of Bacillus amyloliquefaciens MBI600[J]. Msphere, 2021, 6(4): 10-1128. [67] Pirhonen M, Flego D, Heikinheimo R, et al. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora[J]. EMBO Journal, 1993, 12(6): 2467-2476. [68] Krzyzanowska DM, Potrykus M, Golanowska M, et al. Rhizosphere bacteria as potential biocontrol agents against soft rot caused by various Pectobacterium and Dickeya spp. strains[J]. Journal of Plant Pathology, 2012, 94(2): 367-378. [69] Yu Y, Gui Y, Li Z, et al. Induced systemic resistance for improving plant immunity by beneficial microbes[J]. Plants-Basel, 2022, 11(3): 386. [70] Ongena M, Jourdan E, Adam A, et al. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants[J]. Environmental Microbiology, 2007, 9(4): 1084-1090. [71] Huang C J, Tsay J F, Chang S Y, et al. Dimethyl disulfide is an induced systemic resistance elicitor produced by Bacillus cereus C1L[J]. Pest Management Science, 2012, 68(9): 1306-1310. [72] Tsotetsi T, Nephali L, Malebe M, et al. Bacillus for plant growth promotion and stress resilience: what have we learned?[J]. Plants-Basel, 2022, 11(19): 2482. [73] Shao M-W, Chen H-J, Huang A-Q, et al. Modulation of rhizosphere microbiota by Bacillus subtilis R31 enhances long-term suppression of banana Fusarium wilt[J]. iMetaOmics, 2025, 2(2): e70006. [74] Liu Y, Zhang H, Wang J, et al. Nonpathogenic Pseudomonas syringae derivatives and its metabolites trigger the plant “cry for help” response to assemble disease suppressing and growth promoting rhizomicrobiome[J]. Nature Communications, 2024, 15(1): 1907. [75] Li J, Wu L, Zhou Y, et al. Kobresia humilis via root-released flavonoids recruit Bacillus for promoted growth[J]. Microbiological Research, 2024, 287: 127866. [76] Guo L, Zhang X, Zhao J, et al. Enhancement of sulfur metabolism and antioxidant machinery confers Bacillus sp. Jrh14-10–induced alkaline stress tolerance in plant[J]. Plant Physiology and Biochemistry, 2023, 203: 108063. [77] Abdelaziz A M, Hashem A H, El-Sayyad G S, et al. Biocontrol of soil borne diseases by plant growth promoting rhizobacteria[J]. Tropical Plant Pathology, 2023, 48(2): 105-127. [78] Teixidó N, Usall J, Torres R. Insight into a successful development of biocontrol agents: production, formulation, packaging, and shelf life as key aspects[J]. Horticulturae, 2022, 8(4): 305. [79] Stojanovic S S, Karabegovic I, Beskoski V, et al. Bacillus based microbial formulations: optimization of the production process[J]. Hemijska Industrija, 2019, 73(3): 169-182. [80] 刘晓辉, 敖静, 高晓梅, 等. 一株拮抗多种土传病害病原菌的芽胞杆菌筛选鉴定及发酵工艺优化[J]. 微生物学杂志, 2025, 45(2): 70-79. [81] 张晓云, 曲远航, 郭庆港, 等. 萎缩芽胞杆菌HMB22922发酵工艺及其制剂研制[J]. 中国生物防治学报, 2019, 35(5): 768-776. [82] Sanchis V. From microbial sprays to insect-resistant transgenic plants: history of the biospesticide Bacillus thuringiensis: A review[J]. Agronomy for Sustainable Development, 2011, 31(1): 217-231. |
| [1] | 束长龙, 彭东海, 曹蓓蓓, 谢家健, 张杰. 我国苏云金芽胞杆菌研究应用发展历程与展望[J]. 中国生物防治学报, 2026, 42(2): 278-291. |
| [2] | 李亚红, 姚力铭, 陈心宇, 王科晶, 王斌, 蒋先芝, 刘文德, 赵长江, 吴瀚翔. 复合芽孢杆菌种衣剂对玉米茎腐病的防治及促生作用[J]. 中国生物防治学报, 2026, 42(1): 1-9. |
| [3] | 周建波, 孟庆格, 赵彤, 赵岩, 曹玉茹, 孔楷水, 李捷. 两种芽胞杆菌联合使用对黄花菜三线镰孢叶斑病的防控作用[J]. 中国生物防治学报, 2026, 42(1): 64-71. |
| [4] | 胡尊瑞, 段彦丽, 展昭凤, 高乐, 汤久杨, 王振营, 吴晓云, 田家铭. 赤眼蜂不同品系对亚洲玉米螟的田间防治效果[J]. 中国生物防治学报, 2026, 42(1): 134-142. |
| [5] | 杨光源, 张轲, 王广, 蔡浩升, 李政宾, 谢永辉, 茶陈文, 顾小飞, 陈斌. 一株侵染斜纹夜蛾的核型多角体病毒的分离鉴定及毒力分析[J]. 中国生物防治学报, 2026, 42(1): 208-217. |
| [6] | 贺斌琪, 杜立新, 李子煦, 侯晓强, 刘晓微, 纪莉景. 贝莱斯芽胞杆菌的分离鉴定及对白术根腐病的防病促生效果[J]. 中国生物防治学报, 2025, 41(6): 1359-1368. |
| [7] | 阮宏椿, 黄艳琴, 贾孟, 徐婷, 兰成忠. 链霉菌FJ-10的鉴定及其对大豆炭疽病的防效[J]. 中国生物防治学报, 2025, 41(6): 1369-1378. |
| [8] | 王艳平, 张凯, 贾景丽, 郑玉宝, 贾立君, 赵静陶, 王凤鹭, 李乾, 毛海岩, 张崇, 杨宇, 吴元华. 马铃薯疮痂病生防菌贝莱斯芽胞杆菌Apcs-1鉴定及促生防病特性[J]. 中国生物防治学报, 2025, 41(6): 1390-1402. |
| [9] | 王艺璇, 柳敬龙, 李惠霞, 费芍丹, 孙佳聪, 任兴平, 田晓明, 刘永刚, 张海英. 生防副地衣芽孢杆菌ZYGT1811的GFP标记及其在小麦植株和根际土的定殖动态[J]. 中国生物防治学报, 2025, 41(6): 1403-1411. |
| [10] | 肖畑, 高雨欣, 郭开发, 金晨钟, 郝亚伦. 农用酵素对油茶炭疽病的防治效果[J]. 中国生物防治学报, 2025, 41(6): 1430-1438. |
| [11] | 彭宇, 胡亚杰, 吴惠平, 范啟铃, 李彩斌, 唐艾, 陈德慧, 李想, 陈建国, 丁婷, 吴峰. 枯草芽胞杆菌GXHZ16对烟草黑胫病的生防潜力[J]. 中国生物防治学报, 2025, 41(6): 1439-1451. |
| [12] | 安琦, 肖俊博, Clement Nzabanita, 盛雪渊, 王双超, 郭立华, 赵彦. 锐顶镰孢菌G3-23对几种植物病原真菌的生防作用[J]. 中国生物防治学报, 2025, 41(6): 1452-1461. |
| [13] | 任乃芃, 刘杰淋, 郝宁科, 曹阳, 李国良, 刘香萍. 雅致放射毛霉AD-G14对紫花苜蓿镰刀菌根腐病的防效[J]. 中国生物防治学报, 2025, 41(6): 1462-1472. |
| [14] | 高海燕, 曹时瑾, 白文斌, 郝佳丽, 张建华. 烟嘧磺隆降解菌弯曲假单胞菌XZ4的降解特性与作用机制研究[J]. 中国生物防治学报, 2025, 41(6): 1473-1484. |
| [15] | 王恩东, 闫红, 张博, 徐学农. 捕食螨在蔬菜生产中的应用[J]. 中国生物防治学报, 2025, 41(6): 1494-1508. |
| 阅读次数 | ||||||
|
全文 |
|
|||||
|
摘要 |
|
|||||
版权所有 © 《中国生物防治学报》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发
技术支持:support@magtech.com.cn