Abstract: Under constant temperature and humidity conditions, fecundity, developmental duration and survival rate were compared between Trichogramma dendrolimi infected and uninfected with Wolbachia using the staining method by Safranin in egg of Antheraea pernyi. Results show that the fecundity was not significantly reduced by infection with Wolbachia. Developmental durations of pre-pupal and late pupal stages (1.9 and 1.4 d, respectively) of T. dendrolimi infected with Wolbachia were significantly longer than those of uninfected wasps (0.9 and 0.5 d, respectively). Wolbachia infection significantly decreased survival rates of eggs and larvae of T. dendrolimi.