Binding Specificity Analysis of Odorant Binding Protein AlucOBP8 of Apolygus lucorum (Meyer-Dür)

doi:10.16409/j.cnki.2095-039x.2015.06.002

Abstract

Abstract: Odorant binding proteins (OBPs), an important class of proteins, are involved in the process of insect olfactory perception. One of OBP’s functions is to bind and transport odor molecules through the lymph to reach neuronal dendrites. In the present study, we cloned an OBP gene of Apolygus lucorum (Meyer-Dür) (GenBank No.JQ675725) and named AlucOBP8. The quantitative real-time PCR (qRT-PCR) analysis demonstrated that the AlucOBP8 was mainly expressed in the antennae of both male and female adult bugs. The fluorescence competitive binding assays were conducted to measure the binding abilities of recombinant AlucOBP8 to seven sex pheromone analogs and thirty-three plant volatiles. The results revealed that AlucOBP8 could not bind to sex pheromone analogs. Only two plant volatiles, α-caryophyllene and dodecyl aldehyde showed strong binding affinities and the dissociation constant were 8.74 and 9.99 μmol/L, respectively. We measured the taxis selection of A. lucorum to α-caryophyllene and dodecyl aldehyde by using Y tube olfactometer. The results showed that dodecyl aldehyde had significantly repellent action on A. lucorum adult males. We suspected that the AlucOBP8 might be a general odorant binding protein to play roles in odor recognition of host plant volatiles.

Key words: Apolygus lucorum, AlucOBP8, plant volatiles, binding assay, taxis selection

CLC Number:

S436.421

ZHU Xiaoqiang, DING Yuxiao, LIU Hangwei, ZHOU Yanle, ZHANG Yongjun, GUO Yuyuan. Binding Specificity Analysis of Odorant Binding Protein AlucOBP8 of Apolygus lucorum (Meyer-Dür)[J]. journal1, 2015, 31(6): 821-829.

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