Screening of Beauveria bassiana Strains against Frankliniella occidentalis and Their Conidial Thermotolerance

doi:10.16409/j.cnki.2095-039x.2015.06.005

Abstract

Abstract: In the test of 191 isolates of Beauveria bassiana for their virulence against adult Frankliniella occidentalis, 61 isolates displayed high virulence and LT₅₀ less than 4.1 d. The conidial suspension of each screened isolate was exposed to the thermal stress of 48 °C in a water bath. LT₅₀, the time for 50% of conidia to lose viability under thermal stress, was defined as a thermotolerance index. The results showed that the median LT₅₀ of the 61 isolates were 34.28 min (6.94—53.67 min), and 10 isolates were determined as high thermotolerance strains, whose LT₅₀ were longer than 45 min. These 10 isolates were further subcultured for five generations separately, and then the conidia were exposed to thermal stress of 48 ℃. As a result, thermotolerance of 6 isolates showed degeneration, while the other 4 isolates, XJWLMQ-49, XJWLMQ-32, JLGZL-14 and SCWJ-9, maintained stable thermotolerance. These 4 isolates could be regarded as biological control strains with potential for development and application.

Key words: Frankliniella occidentalis, Beauveria bassiana, thermotolerance, subculture, degeneration

CLC Number:

S476.12

LI Juan, WU Shengyong, WANG Xiaoqing, WANG Shuaiyu, LEI Zhongren. Screening of Beauveria bassiana Strains against Frankliniella occidentalis and Their Conidial Thermotolerance[J]. journal1, 2015, 31(6): 845-852.

References

[1] 农向群, 高松, 邓春生, 等. 白僵菌绿僵菌分生孢子对高温的耐受力[J]. 中国生物防治, 1999, 15(3): 111-114.
[2] Feng M G, Poprawski T J, Khachatourians G G. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: current status[J]. Biocontrol Science and Technology, 1994, 4(1): 3-34.
[3] 王成树, 王四宝, 樊美珍, 等. 球孢白僵菌菌株耐热力与贮藏稳定性的关系[J]. 中国生物防治, 1999, 15(4): 162-165.
[4] Luz C, Fargues J. Temperature and moisture requirement for conidial germination of an isolate of Beauveria bassiana, pathogenic to Rhodnius prolixus[J]. Mycopathology, 1997, 138(3): 117-125.
[5] 李银平, 雷仲仁, 王海鸿. 对西花蓟马高效的球孢白僵菌菌株筛选及产孢特性研究[J]. 中国生物防治学报, 2013, 29(2): 219-226.
[6] 张友军, 吴青君, 徐宝云, 等. 危险性外来入侵生物—西花蓟马在北京发生危害[J]. 植物保护, 2003, 29(4): 58-59.
[7] 俞佳, 冯明光. 基于分生孢子热胁迫反应的球孢白僵菌耐热菌株筛选[J]. 菌物学报, 2006, 25(2): 278-283.
[8] McClatchie G V, Moore D, Bateman R P, et al. Effects of temperature on the viability of the conidia of Metarhizium flavoviride in oil formulations[J]. Mycological Research, 1994, 98(7): 749-756.
[9] Wraight S P, Jackson M A, de Kock S L. Production, stabilization and formulation of fungal biological agents//Butt T, Jackson C, Magan N. eds. Fungi as Biocontrol Agents: Progress, Problems and Potential[M]. London: CAB International, 2001, 253-287.
[10] 张志光, 李建宗, 欧笑兰, 等. 白僵菌生活史的研究(II)异核体的形成和准性生殖的显微摄影观察[J].湖南师院学报(自然科学版), 1981, (2): 38-44.
[11] 王静, 雷仲仁, 徐洪富, 等. 白僵菌对西花蓟马若虫的致病力和对巴氏钝绥螨的影响[J].中国生物防治学报, 2011, 27(4): 479-484.
[12] Ying S, Feng M. Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents[J]. Journal of Applied Microbiology, 2004, 97: 323-331.
[13] 李鸿文, 冯明光. 球孢白僵菌不同菌株分生孢子的耐热能力[J].浙江大学学报(农业与生命科学版), 2008, 34(2): 158-162.