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journal1 ›› 2016, Vol. 32 ›› Issue (1): 1-12.DOI: 10.16409/j.cnki.2095-039x.2016.01.001

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Sequence Analysis on Genes Region from COⅡ to COⅢ in mtDNA of Several Sclerodermus Species in China for Biological Control

JIANG Yuan1,2, YANG Zhongqi2, WANG Xiaoyi2, WEI Ke2, ZHANG Yinan1, LI Zhiqiang1, WU Xiaoyun1   

  1. 1. Beijing Vocational College of Agriculture, Beijing 102442, China;
    2. Key Laboratory of Forest Protection of State Forestry Administration/Research Institute of Forest Ecology, Chinese Academy of Forestry, Beijing 100091, China
  • Received:2015-01-12 Online:2016-02-08 Published:2016-02-22

Abstract: Several species of genus Sclerodermus are currently the most important natural insect enemies against forest wood borer pests, mainly buprestid and cerambycid beetles. We aimed to use molecular methods to identify Sclerodermus species that are difficult to distinguish morphologically. Further, we provided a reliable evidence for traditional morphological identification. Sequence analysis was on genes region from COⅡ to COⅢ in mtDNA of several Sclerodermus species used in biological control in recent years. The sequence contained partial mtDNA COⅡ, tRNA-Asp, tRNA-Lys, ATPase6, ATPase8 and part COⅢ, measuring about 1700 bp. Sequence similarity between S. guani and S. sichuanensis was 100%, while it was 96.88% between S. pupariae and Sclerodermus sp. (No. 2). The 1700 bp region of mtDNA COⅡ-COⅢ was obtained for the 106 individuals, including 68 variable sites and sequence divergences (K2P) of 0.011. NJ analysis of the mtDNA COⅡ-COⅢ sequences revealed that the S. pupariae were merged into one haplogroup, and Sclerodermus sp. (No. 1) were merged into another haplogroup. The sequences of Sclerodermus mtDNA COⅡ-COⅢ were first reported in this paper. The comparative analysis results of different mtDNA sequences show that the ATPase6 gene is the most suitable for distinguishing the sibling species of Sclerodermus.

Key words: Sclerodermus, mtDNA, sequence analysis, molecular evolution

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