Sequence Analysis on Genes Region from COⅡ to COⅢ in mtDNA of Several Sclerodermus Species in China for Biological Control

doi:10.16409/j.cnki.2095-039x.2016.01.001

Abstract

Abstract: Several species of genus Sclerodermus are currently the most important natural insect enemies against forest wood borer pests, mainly buprestid and cerambycid beetles. We aimed to use molecular methods to identify Sclerodermus species that are difficult to distinguish morphologically. Further, we provided a reliable evidence for traditional morphological identification. Sequence analysis was on genes region from COⅡ to COⅢ in mtDNA of several Sclerodermus species used in biological control in recent years. The sequence contained partial mtDNA COⅡ, tRNA-Asp, tRNA-Lys, ATPase6, ATPase8 and part COⅢ, measuring about 1700 bp. Sequence similarity between S. guani and S. sichuanensis was 100%, while it was 96.88% between S. pupariae and Sclerodermus sp. (No. 2). The 1700 bp region of mtDNA COⅡ-COⅢ was obtained for the 106 individuals, including 68 variable sites and sequence divergences (K2P) of 0.011. NJ analysis of the mtDNA COⅡ-COⅢ sequences revealed that the S. pupariae were merged into one haplogroup, and Sclerodermus sp. (No. 1) were merged into another haplogroup. The sequences of Sclerodermus mtDNA COⅡ-COⅢ were first reported in this paper. The comparative analysis results of different mtDNA sequences show that the ATPase6 gene is the most suitable for distinguishing the sibling species of Sclerodermus.

Key words: Sclerodermus, mtDNA, sequence analysis, molecular evolution

CLC Number:

S476.3

JIANG Yuan, YANG Zhongqi, WANG Xiaoyi, WEI Ke, ZHANG Yinan, LI Zhiqiang, WU Xiaoyun. Sequence Analysis on Genes Region from COⅡ to COⅢ in mtDNA of Several Sclerodermus Species in China for Biological Control[J]. journal1, 2016, 32(1): 1-12.

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