The Sequence and Function Analysis of AVR-Pita Gene Family of Rice Blast Fungus, Magnaporthe oryzae in Philippines

doi:10.16409/j.cnki.2095-039x.2018.03.006

Abstract

Abstract: For controlling rice blast and providing theory for breeding and selecting resistant rice variety, we studied the sequence polymorphism and function of avirulence gene AVR-Pita homologs. According to the cloned AVR-Pita homologs, we designed 4 primer pairs, selected 74 isolates from Philippine and distracted their DNA as template for PCR. The results showed that 42 isolates had the band of AVR-Pita1 gene, 31 isolates had the band of AVR-Pita3 gene, and 55 isolates had the band of AVR-Pita4 gene. However, no isolates showed the band of AVR-Pita2 gene. Sequences analysis of PCR products showed there were 5 haplotypes among AVR-Pita1 gene with variation in 10 base sites, while there was no polymorphic base site in AVR-Pita3 and AVR-Pita4 genes, indicating both genes were conservative. Inoculation tests demonstrated that IRBLta-CT2 was susceptible after inoculated with the haplotype 1, 2, 4 and 5, except haplotype 3. In conclusion, AVR-Pita1 gene is of strong variation, and its function will change after gene sequence has changed.

Key words: rice blast fungus, AVR-Pita gene family, sequence analysis, function analysis

CLC Number:

S435.11

GAN Yuzi, XIAO Gui, DENG Qiyun, WU Jun, BAI Bin, LU Xiangyang, ZHOU Bo. The Sequence and Function Analysis of AVR-Pita Gene Family of Rice Blast Fungus, Magnaporthe oryzae in Philippines[J]. journal1, 2018, 34(3): 488-498.

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