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journal1 ›› 2018, Vol. 34 ›› Issue (3): 488-498.DOI: 10.16409/j.cnki.2095-039x.2018.03.006

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The Sequence and Function Analysis of AVR-Pita Gene Family of Rice Blast Fungus, Magnaporthe oryzae in Philippines

GAN Yuzi1,2,3, XIAO Gui2,3, DENG Qiyun2, WU Jun2, BAI Bin2, LU Xiangyang1, ZHOU Bo3   

  1. 1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Hybrid Rice Research Center, Changsha 410125, China;
    3. Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, Manila 0900, Philippines
  • Received:2017-12-12 Online:2018-06-08 Published:2018-06-14

Abstract: For controlling rice blast and providing theory for breeding and selecting resistant rice variety, we studied the sequence polymorphism and function of avirulence gene AVR-Pita homologs. According to the cloned AVR-Pita homologs, we designed 4 primer pairs, selected 74 isolates from Philippine and distracted their DNA as template for PCR. The results showed that 42 isolates had the band of AVR-Pita1 gene, 31 isolates had the band of AVR-Pita3 gene, and 55 isolates had the band of AVR-Pita4 gene. However, no isolates showed the band of AVR-Pita2 gene. Sequences analysis of PCR products showed there were 5 haplotypes among AVR-Pita1 gene with variation in 10 base sites, while there was no polymorphic base site in AVR-Pita3 and AVR-Pita4 genes, indicating both genes were conservative. Inoculation tests demonstrated that IRBLta-CT2 was susceptible after inoculated with the haplotype 1, 2, 4 and 5, except haplotype 3. In conclusion, AVR-Pita1 gene is of strong variation, and its function will change after gene sequence has changed.

Key words: rice blast fungus, AVR-Pita gene family, sequence analysis, function analysis

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