Growth-promotion Effect of Bacillus amyloliquefaciens B1619 on Tomato Plant

doi:10.16409/j.cnki.2095-039x.2016.03.010

Abstract

Abstract: Bacillus amyloliquefaciens B1619 is a biological control bacterial strain which has significant efficiency to control soil-borne diseases of tomato in greenhouse cultivation. For defining the role of B1619 strain in the growth of tomato, the physiologcal and biochemical characteristics, growth of tomatoes chlorophylls, carotenoids and root activity of tomato treated with different dilutions supernatant of B1619 were investigated. The HPLC-MS methods were established for the determination of bioactive substances (auxin, cytokinin, abscisic acid, gibberellin and sperdimine) in supernatant of B1619 strain. The results showed that biocontrol bacterial strain B1619 with different dilutions supernatant could promote seed germination and seeding growth of tomato and the 100 times dilution supernatant (4.8×10⁷ cfu/mL) of B1619 strain was the best. The contents of chlorophyll in leaves and the root activity of plant were increased in varying degrees after spraying the biocontrol B1619 with the different dilutions supernatant, the highest of which was 100 times dilution (increased by 47.92% in total chlorophyll, 38.37% in carotenoid and 49.75% in root activity). The content of auxins (IAA), cytokinins (CTK), gibberellins (GA₃), abscisic acid (ABA) and spermidine (Spd) was 1.90, 1.30, 0.0544, 1.94, 1.22 mg/L respectively. As a consequence, bacterium strain B1619 strain can significantly improve the growth and development of tomato plant, and the promotion factor existed mainly in the supernatant.

Key words: Bacillus amyloliquefaciens B1619, chlorophyll, root activity, growth-promoting substances

CLC Number:

S476

YANG Xiaoyun, CHEN Zhiyi, JIANG Panpan, ZHANG Rongsheng, LIU Youzhou, LIU Yongfeng. Growth-promotion Effect of Bacillus amyloliquefaciens B1619 on Tomato Plant[J]. journal1, 2016, 32(3): 349-356.

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