Population Dynamics of Trichoderma spp., Bacillus subtillis and Fusarium solani f.sp.cucurbitae in Cucumber Rhizosphere

doi:10.16409/j.cnki.2095-039x.2016.03.011

Abstract

Abstract: The biocontrol efficiency of Trichoderma spp. and Bacillus subtilis on cucumber root rot caused by Fusarium solani f. sp. cucurbitae and their quantitative dynamic changes in cucumber rhizosphere soil were monitored. Results showed that application of bio-agents reduced significantly the disease incidence of cucumber root rot and control efficiency reached 100% and 92.5%, 96.71% and 86.76%, 85.35% and 79.24% at 7, 14 and 27 days after treatment with 500×Trichoderma spp. bio-organic fertilizer and 100× B. subtilis, respectively. While the copy numbers from Trichoderma spp. and B. subtilis decreased after 7 days of inoculation from 130787 copys/μL) to 51161 copys/μL, and increased rapidly later maintained up to 295139 copys/μL and 680556 copys/μL from 7 d to 28 d for B. subtillis. The change tendency of Trichoderma spp. was the same as the B. subtilis. On the other hand, the copy numbers of the pathogen, F. solani, in cucumber rhizosphere, increased after 7 days of inoculation from 2.61 copys/μL up to 11.22 copys/μL when treated with B. subtilis and 15.34 copys/μL to 20.98 copys/μLwith Trichoderma spp., respectively. And then, maintained at the high level for a period of time. Thedynamic change of B. subtilis, Trichoderma spp. and F. solani detected with plate dilution method was similar to that with RT-PCR.

Key words: Trichoderma spp., Bacillus subtilis, Fusarium solani f.sp.cucurbitae, real-time fluorescent quantitative PCR, dynamic change detection

CLC Number:

S476

HE Zidian, WU Suxia, SONG Xiaofei, XIE Xinyu, LI Shidong. Population Dynamics of Trichoderma spp., Bacillus subtillis and Fusarium solani f.sp.cucurbitae in Cucumber Rhizosphere[J]. journal1, 2016, 32(3): 357-364.

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