Screening, Identification and Biocontrol Effects of Antagonistic Bacterial Strain CQ against Botrytis cinerea from Tomato

doi:10.16409/j.cnki.2095-039x.2015.06.020

Abstract

Abstract: Bacteria strain CQ was isolated from Yunnan Provence, which exhibited a strong antagonistic activity on mycelia growth of Botrytis cinerea. Affected fungal hyphae formed abnormal branches and bubble deformation structures. The formation of the spores of B. cinerea was inhibited in agar medium amended with 1‰ cultural filtrate of the strain. Control efficacies on tomato gray mold disease caused by B. cinerea were 100%, when detached tomato leaves were sprayed with 1‰ CQ cultural filtrate of the strain; the incidence decreased by 28.5%, 21.3%, and the disease index decreased by 44.01, 37.86 respectively, when using its 10 times and 100 times dilution filtrate sprayed on tomato seedlings. The strain CQ was identified as Bacillus subtilis according to its biochemical characteristics.

Key words: Botrytis cinerea, Bacillus subtilis, identification, biocontrol

CLC Number:

S476

YANG Limin, TONG Zanhua, GUO Lihua, JIANG Xiliang, QIU Dewen, LI Miao. Screening, Identification and Biocontrol Effects of Antagonistic Bacterial Strain CQ against Botrytis cinerea from Tomato[J]. journal1, 2015, 31(6): 956-960.

References

[1] 刘京涛, 刘庆年, 赵辉, 等. 防治番茄灰霉病新药剂筛选试验//中国植物病理学会第七届代表大会暨学术研讨会论文摘要集[C]. 2002.
[2] 葛绍荣, 牛莉娜, 李铭. 番茄灰霉病害及其微生物防治的研究进展[J]. 生物加工过程, 2007, 5(3): 15-19.
[3] 纪军建, 张小风, 王文桥, 等. 番茄灰霉病防治研究进展[J]. 中国农学通报, 2012, 28(31): 109-113.
[4] Kretschmer M, Hahn M. Fungicide resistance and genetic diversity of Botrytis cinerea isolates from a vineyard in Germany[J]. Journal of Plant Disease and Protection, 2008, 115(5): 214-219.
[5] Myresiotis C K, Karaoglandis G S, Tzavella-Klonari K. Resistangce of Botrytis cinerea isolates from vegetable crops to Anilinopyrimidine, Phenyrrole, Hydroxyanilide, Benzimidazole, and Dicarboximide fungicides[J]. Plant Disease, 2007, 91(4): 407-413.
[6] Moyano C. Gomez V. Melgarejo P . Resistance topyrimethanil and other fungicides in Botrytis cinerea populations collected on vegetable crops in Spain [J]. Journal of phytopathology, 2004, 152(8-9): 484-490.
[7] Daniel A N, Ursula K, Lukas S, et al. Dual antagonism of aldehydes and epiphytic bacteria from strawberry leaf surfaces against the pathogenic fungus Botrytis cinerea in vitro[J]. BioControl, 2006, 51(3): 279-291.
[8] Estibaliz S, Candelario V, Aurelio O. Genetic manipulation in Bacillus thuringiensis for strain improvement[J]. 2010, 32(11): 1549-1557.
[9] Guinebretiere M H, Nguyen-The C, Morrison N, et al. Isolation and characterization of antagonists for the control of the post harvest wound pathogen Botrytis cinerea on strawberry fruits[J]. Journal of Food Protection, 2000, 63(3): 386-394.
[10] Montealegre J R, Reyes R, Perez LM, et al. Selection of bioantagonistic bacteria to be used in biological control of Rhizoctonia solani in tomato[J]. Electronic Journal of Biotechnology, 2003, 6(2): 115-127.
[11] 钟卫鸿. 基因工程技术实验指导[M]. 北京: 化学工业出版社, 2007.
[12] Galkiewicz J P, Kellogg C A. Cross-kingdom amplification using Bacteria-specific primers: complications for studies of coral microbial ecology[J]. Applied and Environmental Microbiology, 2008, 74(24): 7828-7831.
[13] 童蕴慧, 纪兆林, 徐敬友, 等. 灰葡萄孢拮抗细菌的种类鉴定及生长条件研究[J]. 扬州大学学报, 2002, 23(2): 67-70.
[14] Klein T A, Burgess L W, Ellison F W. The incidence and spatial patterns of wheat plants infectd by Fusariym graminearum group land the effect of crown roton yield[J]. Australian Journal of Agricultural Research, 1991, 42(3): 399-407.
[15] 张玉勋, 李光, 张光明. 拮抗细菌在大棚温室番茄叶片定殖及对灰霉病害的控制效果[J]. 植物病理学报, 2000, 30(1): 91.