Screening, Resistance and Growth Promoting of Antagonistic Endophytic Bacteria Isolated from Root Nodules of Soybean against Ascochyta glycines

doi:10.16409/j.cnki.2095-039x.2016.03.016

Abstract

Abstract: Two hundred and seventy-six endophytic bacteria isolated from soybean root nodules of different areas in Henan province were screened by confrontation culture and fermentation filtrate culture methods for strains with strong inhibitory effect on Ascochyta glycines Miura, and morphological changes of phytopathogen hyphae was examined by microscopy. Their phylogenetic relationship was determined by homology analysis of the 16S rDNA sequences of PCR products, physiological and biochemical tests and 16S rDNA sequence analysis. In addition, the salt and alkali resistance assay and inoculation tests were conducted for representative strain. The results showed that seventeen antagonistic strains were verified by secondary screening and inhibition activity of fermentation filtrate methods, and 8 stronger antagonistic strains showed more than 50% inhibitory effect. The inhibited phytopathogen hyphae became mutual distorted, wrapped into a ring, terminal hyphae became branch increase, thin, transparent, and partially expanded forming protoplasm concentration and fracture, some hyphae were embedded by forming biofilms, gradually melted. Five (DD013, DD123, DD156, DD159, DD287) of eight endophytes were preliminary identified as Bacillus, DD150 as Lysinibacillus, DD267 as Enterobacter, DD303 as Ochrobactrum, respectively. The results of salt and alkali resistance and growth curve of strain with stronger inhibition effect showed that DD287 has certain resistance to 3% salt concentration, and can normally grow under alkaline environment (pH8), while affected by increasing pH (9~11). Some antagonistic strains here promoting effect on soybean growth. For screened antagonistic strains, it is of great significance to rich biological control strain resources.

Key words: Ascochyta glycines, root nodule endophyte, antagonism, salt and alkali resistance, 16S rDNA sequence

CLC Number:

S476

ZHAO Longfei, XU Yajun, CHANG Jiali. Screening, Resistance and Growth Promoting of Antagonistic Endophytic Bacteria Isolated from Root Nodules of Soybean against Ascochyta glycines[J]. journal1, 2016, 32(3): 396-405.

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