Antagonism of Bjerkandera adusta M-1 against Colletotrichum gloeosprioides and Optimization of Fermentation Conditions

doi:10.16409/j.cnki.2095-039x.2018.03.010

Abstract

Abstract: In this study, the biocontrol efficiency of Bjerkandera adusta M-1 strain on Colletotrichum gloeosprioides was investigated by the plate confrontation test and fermentation conditions of M-1 were optimized by the single factor test and the response surface methodology. The results showed that M-1 can obviously inhibit the growth of C. gloeosprioides, with the inhibitory rate of 65.7%, significantly higher than Carbendazim and Thiophanate methyl. The optimized fermentation conditions were C/N of 7.5, pH of 6.5, culture volume 36% of bottling capacity, a culture time of 22 d, a rotational speed of 180 r/min, and a culture temperature of 30℃, and the inhibition rate reached 71.1%. In conclusion, it was the first report that B. adusta M-1 strain had a strong antagonistic activity against C. gloeosprioides, and the inhibition rate of fermentation broth was strengthened after the fermentation parameters were optimized. The results laid a theoretical foundation for potential application of M-1 against C. gloeosprioides in practice.

Key words: Bjerkandera adusta, response surface methodology, Colletotrichum gloeosprioides, fermentation broth, antagonism

CLC Number:

S476

WANG Wenqiang, LI Yong, ZHANG Xuhui, WEI Wanling, ZHOU Fengwu, LI Suping, LI Jie. Antagonism of Bjerkandera adusta M-1 against Colletotrichum gloeosprioides and Optimization of Fermentation Conditions[J]. journal1, 2018, 34(3): 423-430.

References

[1] Timmer L W, Agostini J P, Zitko S E, et al. Postbloom fruit drop, an increasingly prevalent disease of citrus in the Americas[J]. Plant Disease, 1994, 78(4):329.
[2] 王卫芳, 冷怀琼. 柑桔炭疽病潜伏带菌的普遍性及侵染动态[J]. 四川农业大学学报, 1990, 8(2):147-151.
[3] 焦雁翔, 王日葵, 胡军华, 等. 重庆及周边部分地区柑橘炭疽病菌鉴定[J]. 果树学报, 2014, 31(5):893-900.
[4] Lima W G, Spósito M B, Amorim L, et al. Colletotrichum gloeosporioides, a new causal agent of citrus post-bloom fruit drop[J]. European Journal of Plant Pathology, 2011, 131(1):157-165.
[5] 马艳, 李艳霞, 常志州, 等. 强化拮抗菌有机肥在连作大棚草莓上的应用效果研究[J]. 植物营养与肥料学报, 2011, 17(6):1459-1467.
[6] 王元乐, 黄小军, 谢维. 早熟抗病苦瓜新品种潭白一号的选育[J]. 长江蔬菜, 2011(6):15-17.
[7] 汪茜, 胡春锦, 柯仿钢, 等. 柑橘炭疽病生物防治研究进展[J]. 南方农业学报, 2010, 41(4):341-344.
[8] Saizurra L, Bustillo Pérez A J, Cruzmonteagudo M, et al. Global antifungal profile optimization of chlorophenyl derivatives against botrytis cinerea and colletotrichum gloeosporioides[J]. Journal of Agricultural and Food Chemistry, 2009, 57(11):4838-4843.
[9] 尹大川, 祁金玉, 邓玉侠, 等. 两种杀菌剂对立枯丝核菌生长的抑制作用机理[J]. 沈阳农业大学学报, 2017, 48(1):94-100.
[10] 杜云英. 甜菜立枯病防治研究进展[J]. 中国糖料, 2009(2):55-57.
[11] Heydari A, Misaghi I J. The role of rhizosphere bacteria in herbicide-mediated increase in Rhizoctonia solani-induced cotton seedling damping-off[J]. Plant and Soil, 2003, 257(2):391-396.
[12] 汪茜, 胡春锦, 柯仿钢, 等. 生防菌株1404的鉴定及其对采后柑橘炭疽病的防治效果[J]. 微生物学报, 2010, 50(9):1208-1217.
[13] 刘冰, 李恩, 乐美玲, 等. 1株柑橘病害生防细菌鉴定、发酵条件优化及田间防效测定[C]//中国植物病理学会2015年学术年会论文集, 2015.
[14] Jackson A M, Whipps J M, Lynch J M. Nutritional studies of four fungi with disease biocontrol potential[J]. Enzyme and Microbial Technology, 1991, 13(6):456-461.
[15] Kredics L, Antal Z, Manczinger L, et al. Influence of environmental parameters on Trichoderma strains with biocontrol potential[J]. Food Technology and Biotechnology, 2003, 41(7-8):37-42.
[16] 彭滟钞. 烟色烟管菌诱变提高漆酶产量的研究[D]. 长沙:中南林业科技大学, 2014.
[17] Iwahara K, Honda Y, Watanabe T, et al. Polymerization of guaiacol by lignin-degrading manganese peroxidase from Bjerkandera adusta in aqueous organic solvents.[J]. Applied Microbiology and Biotechnology, 2000, 54(1):104-111.
[18] Bak W C. Characteristics of bed-log of shiitake damaged by Bjerkandera adusta and antagonism between these two fungi[J]. The Korean Journal of Mycology, 2011, 39(1):44-47.
[19] 张旭辉, 张红楠, 李勇, 等. 抑制西瓜蔓枯病菌的生防真菌筛选、鉴定及发酵条件优化[J]. 中国生物工程杂志, 2017, 37(5):76-86.
[20] Campanile G, Ruscelli A, Luisi N. Antagonistic activity of endophytic fungi towards Diplodia corticola assessed by in vitro and in planta tests[J]. European Journal of Plant Pathology, 2007, 117(3):237-246.
[21] 崔西苓. 耐盐碱木霉菌株的筛选鉴定、抗病促生及耐盐机理研究[D]. 北京:中国农业科学院, 2014.
[22] 田淑慧, 张启林, 杜宜新, 等. 拮抗立枯丝核菌的木霉菌株筛选[J]. 仲恺农业工程学院学报, 2006, 19(1):14-17.
[23] He Z, Wang J, Oh J, et al. Robust optimization for multiple responses using response surface methodology[J]. Applied Stochastic Models in Business and Industry, 2010, 26(2):157-171.
[24] 蓝星杰. 两种生防菌田间防病促生作用及其机理研究[D]. 杨凌:西北农林科技大学, 2015.
[25] Liu B, Qiao H, Huang L, et al. Biological control of take-all in wheat by endophytic Bacillus subtilis E1R-j and potential mode of action[J]. Biological Control, 2009, 49(3):277-285.
[26] 于晓庆, 郗丽君, 刘永光, 等. 洋葱伯克霍尔德氏菌株Lyc2的鉴定及对棉苗的防病促生作用[J]. 植物病理学报, 2007, 37(4):426-432.
[27] Nasini G, Arnone A, Assante G, et al. Secondary mould metabolites of Cladosporium tenuissimum, a hyperparasite of rust fungi[J]. Phytochemistry, 2004, 65(14):2107.
[28] 雷萍, 吴亚召, 张文隽, 等. 不同搅拌转速和通气量对桑黄深层发酵培养的影响[J]. 食用菌学报, 2014, 21(2):57-62.
[29] 汪华, 喻大昭, 郭坚. 一株多孔烟管菌菌株高氏15号的鉴定及抑菌活性研究[C]//陈万权主编. 病虫害绿色防控与农产品质量安全. 北京:中国农业科学技术出版社, 2015, 590.