Optimization of Genetic Manipulation System for Wuyiencin Producer CK?15

doi:10.16409/j.cnki.2095-039x.2016.04.015

Abstract

Abstract: In order to optimize the genetic manipulation system of Streptomyces ahygroscopicus var. wuyiensis CK-15 for disrupting genes involved in wuyiencin biosynthesis, we developed a method of conjugation to transfer exotic DNA pKC1132 into S. ahygroscopicus var. wuyiensis CK-15. The best protocol of getting mutant by gene knockout was established and a double-crossover mutant of wuyiencin biosynthesis gene was successfully obtained. a stable and efficient genetic manipulation system for S. ahygroscopicus var. wuyiensis CK-15 was developed, which was a solid foundation for the further study of wuyiencin biosynthesis mechanism and more important for combinatorial biosynthesis renovation of wuyiencin.

Key words: wuyiencin, Streptomyces ahygroscopicus var. wuyiensis CK-15, genetic manipulation, pKC1132, gene knockout

CLC Number:

S476

LIU Yan, GE Beibei, LIU Binghua, ZHAO Wenjun, ZHANG Kecheng. Optimization of Genetic Manipulation System for Wuyiencin Producer CK?15[J]. journal1, 2016, 32(4): 524-530.

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