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journal1 ›› 2019, Vol. 35 ›› Issue (1): 53-62.DOI: 10.16409/j.cnki.2095-039x.2019.01.009

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Regulation of the BTXL6_11095 Gene of Bacillus thuringiensis XL6 on Its Biofilm-Related Phenotypes

MA Shenglong1, FAN Xiao1, YAO Junmin1, WU Huachuan1, SHU Changlong2, SHANG Mingda1, ZHANG Shaorui1, YANG Zhenghao1, GUAN Xiong1, HUANG Tianpei1   

  1. 1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Biopesticide and Chemical Biology of Ministry of Education/College of Life Sciences & College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. State Key Laboratory of Plant Diseases and Insect Pests Biology/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2018-08-11 Online:2019-02-08 Published:2019-01-31

Abstract: Under the condition of bacterial biofilm (BBF), bacteria tend to have higher anti-UV-B ability, better environmental adaptability and stronger pesticide resistance. In our previous studies, the BTXL6_11095 gene putatively with the function regulating biofilm formation was screened by transposon insertion into the genome of Bacillus thuringiensis (Bt) XL6 with strong biofilm formation ability. In this study, the potential functions of this gene were analyzed using bioinformatics using BTXL6_11095 knockout mutant and through analysis of the biofilm related phenotypes of the mutant. Bioinformatics analysis showed that BTXL6_11095 gene had the PAS- GGDEF-GGDEF-EAL motif. BTXL6_11095 knockout mutant showed no different growth curve relative to the wild type, but exhibited higher swimming ability, lower biofilm formation ability, and lower resistance to UV-B. This study, through comprehensive evaluation of phenotypic changes of BTXL6_11095 knockout mutant, has revealed the effect of this gene on the biofilm formation ability of Bt XL6, which serves to elucidate biofilm regulation network of Bt XL6 and construct its engineered biofilm with high biofilm formation ability.

Key words: bacterial biofilm, Bacillus thuringiensis, gene knockout mutant, bioinformatic analysis

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