Identification and Expression Analysis on βGRPs of Frankliniella occidentalis

doi:10.16409/j.cnki.2095-039x.2017.02.010

Abstract

Abstract: The objective of this study is to identify the β-1,3-glucan recognition protein in Frankliniella occidentalis to analyse the express model when F. occidentalis infected by the fungusBeauveria bassiana, and to provide a theoretical foundation for clarifying the innate immunity in F. occidentalis. Target genes were screened from the data of high-throughout sequencing, the genes were identified through blasting against the redundancy nucleotide databases when the Evalue was less than 10^-5. Three β-1,3-glucan recognition protein identified in F. occidentalis were performed by protein structure analysis and phylogenetic analysis with homologous genes of other insects. The expression of β-1,3-glucan recognition protein genes of F. occidentalis infected by different active strains of B.bassiana was analyzed by quantitative real-time PCR. Three βGRP genes were identified and designated asFoβGRP1, FoβGRP2, FoβGRP3. The protein structure analysis showed that three sequences had the unique structure of βGRP family which contained conserved domains-glycoside hydrolase activity domain. Homology analysis showed that FoβGRP1 and FoβGRP2 /FoβGRP3 belong to two different clades of FoβGRP. The expression of all the three FoβGRPs peak at 12 h. The expression level of a certain gene is higher when thrips treated by high-activity strain than those treated by low-activity strain. In conclusion, FoβGRPs might be involved in the immune response to B. bassiana. This study provided the foundation to research the function of these genes in the innate immune response in thrips.

Key words: Frankliniella occidentalis, β-1,3-glucan recognition protein, innate immune response

CLC Number:

S476.12

ZHAO Qianqian, ZHANG Tao, WANG Xiaoqi, WANG Haihong. Identification and Expression Analysis on βGRPs of Frankliniella occidentalis[J]. journal1, 2017, 33(2/3): 206-212.

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