Identification and Expression Analysis on Clip Serine Protease Genes of Frankliniella occidentalis

doi:10.16409/j.cnki.2095-039x.2017.01.010

Abstract

Abstract: The study is to identify the clip-domain serine protease genes in Frankliniella occidentalis (FoCSPs), to analyse the express pattern infected by the fungus Beauveria bassiana, and to investigate the function of FoCSPs in innate immunity of F. occidentalis. Nine FoCSPs genes with clip domain were identified in F. occidentalis. Each FoCSP contains a clip domain at the N-terminus and a catalytic domain at the C-terminus. The catalytic domains characterized by 3 conserved amino acids, namely His, Asp and Ser. A phylogenetic tree analysis showed that FoCSP215 and NITRY19 from Nilaparvata lugens were in the same evolutionary group. FoCSP261, FoCSP236, Manduca sexta MsPOAEO, Anopheles gambiae AgCLIP and Manduca sexta NvSP16P in evolution were clustered into one clade. The remaining 6 FoCSPs clustered in one clade, which converge with another branch consisting of 5 MsHPs in M. sexta. After the fungal infection of B. bassiana, the expression level of all FoCSPs in F. occidentalis was up-regulated, although the degrees were different. For each FoCSP, the higher expression level was monitored in infected nymph than in infected adults. In conclusion, FoCSPs might be involved in the immune response to B. bassiana, and therefore could be a potential target for the biological control of F. occidentalis.

Key words: Frankliniella occidentalis, clip serine protease, innate immune response

CLC Number:

S476.12

ZHAO Qianqian, YU Jie, ZHANG Jiliang, WANG Xiaoqi, WANG Haihong. Identification and Expression Analysis on Clip Serine Protease Genes of Frankliniella occidentalis[J]. journal1, 2017, 33(1): 63-69.

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