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journal1 ›› 2017, Vol. 33 ›› Issue (2/3): 281-288.DOI: 10.16409/j.cnki.2095-039x.2017.02.020

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Allelopathic Effects of Fresh Parsley Root Ethanol Extracts on Fusarium oxysporum f. sp. cucumberinum and Allelochemicals Identification

GAO Xiaomin1,2, WANG Jugang1, YUN Xingfu2, BAO Yanyan3   

  1. 1. Institute of South Subtropical Crops Research, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China;
    2. College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China;
    3. Hulunbeir Agricultural Technology Extension and Service Center, Hulunbuir 021008, China
  • Received:2016-08-12 Online:2017-06-08 Published:2017-04-08

Abstract: In field production of cucumber Cucumis sativus, it is relatively difficult to control wilt caused by Fusarium oxysporum f. sp. cucumberinum. However, the extent of infection was reduced by crop rotation with parsley Apium graveloens. To identify inhibitory allelochemicals released into the soil by parsley crop, ethanol extracts from fresh parsley roots were examined after a repeated series of purification (four cycles) in column chromatography using a column with silicone coating. We used a bioassay that incorporated the various column fractions into PDA medium and co-cultured the plates with F. oxysporum. By measuring colony diameter and spores germination rate, we screened the best fractions in terms of allelopathic inhibition effect and identified further allelochemicals of the fourth chromatography using GC-MS. Optimal fractions (RE21101, RE2955, RE6235 and RE6865) obtained from the fourth cycle of chromatography showed considerable allelopathic effect. The allelopathic inhibition effects of the four optimal fractions on hypha growth compared with the fourth cycle tomographic control (CK4), were 54.59%, 52.35%, 51.55% and 50.97%, respectively. In comparison to CK4, the contents of fusaric acid respectively were decreased to 38.09%, 59.54%, 36.78% and 36.65%. Total 16 types allelochemicals, including N,N-dimethyl-1-hexadecanmine, heptadecanoic acid, 1,6-methy-, methyl ester, hexadecanoic acid, 2,4-dimethyl-benzo[b]quinoline, (+)-isomenthol, butylated, hydroxyl, alpha-arabinofuranoside, acetic acid octadecyl ester, 9,12-octadecadienoic acid, 2-hydroxy acid, cis-9-hexadecyl (carbon) acid, fluoren-2-azo-2, 4-dithdroxybenzene, mannitol laurate, menthofuran, 4-menthoxysalicylaldehyde, can be classified into 5 forms matters including organic acids, phenols, alcohols, esters, and nitrogenous compounds, were identified by the GC-MS analysis. The results provided the needed theoretical basis for the prevention and control Fusarium wilt of cucumber by using extracts of parsley.

Key words: parsley fresh root, cucumber wilt, allelopathy, fusaric acid, allelochemicals

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