Elimination of the Inhibition of Fusaric Acid on the Biofilm Formation of Bacillus subtilis R31 by Manganese

doi:10.16409/j.cnki.2095-039x.2019.04.019

Abstract

Abstract: In order to reveal the interaction mechanisms between Bacillus biocontrol agents and plant pathogen Fusarium, the inhibition of fusaric acid (FA) from Fusarium on the biofilm formation of Bacillus subtilis strain R31, and the elimination of the inhibition were investigated. Firstly, a bioassay system was established to test the inhibition of FA on the biofilm formation of B. subtilis R31 by 24-well cell culture plate, and the lowest concentration of FA on the biofilm formation of R31 was screened out. The growth curve of B. subtilis R31 was tested in the presence of the minimum concentration of FA, and the cells of R31 with or without the FA treatment under shaking or static cultivation were observed by microscopy. Then, different concentrations of MnSO₄ were added into the co-cultural system to eliminate the inhibition of FA on the biofilm formation of R31, and the residue of FA in each treatment was determined by HPLC. The results showed that in this bioassay system, 9 μg/mL of FA could drastically inhibit the biofilm formation of R31 by inhibiting its reticular structure formation of R31 cells and reducing the cells floating on the liquid surface in static culture. 9 μg/mL of FA can also inhibit the growth of early-stage R31 in shaking culture. Adding MnSO₄ into the co-culture system, however, can restore the biofilm formation of R31. More specially, 200 μg/mL of MnSO₄ can not only eliminate the inhibition of FA on the biofilm formation of R31, but also significantly promote its formation. FA may inhibit the biofilm formation of R31 by affecting the differentiation of matrix-producing cells of R31, and MnSO₄ can be used as a passivating agent to alleviate the inhibition of FA on the biofilm formation of R31.

Key words: Bacillus subtillis, biofilm formation, fusaric acid, inhibition, elimination

CLC Number:

S476

ZHOU Haiqi, CHENG Ping, GONG Qingyou, YU Guohui, WEN Shuheng. Elimination of the Inhibition of Fusaric Acid on the Biofilm Formation of Bacillus subtilis R31 by Manganese[J]. Chinese Journal Of Biological Control, 2019, 35(4): 613-621.

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