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journal1 ›› 2018, Vol. 34 ›› Issue (3): 377-387.DOI: 10.16409/j.cnki.2095-039x.2018.03.009

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Proteomics Analysis for the Role of Polyphosphate in Lysinibacillus sphaericus Adaptation to Nutrition Recovery

SHI Tingyu1,3, WANG Yuanyuan2, XIANG Yubo1, DONG Xinggao1, Yuan Zhiming3   

  1. 1. Medical College of Hubei Institute for Nationalities, Enshi 445000, China;
    2. Affiliated Hospital of Hubei Institute for Nationalities, Enshi 445000, China;
    3. Key Laboratory of Agricultural and Environmental Microbiology/Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
  • Received:2017-07-21 Online:2018-06-08 Published:2018-06-14

Abstract: Though knocking out the gene ppk of polyphosphate kinase, the growth of Lysinibacillus sphaericus wild strains and ppk mutants in conditions of amino acids deficiency and nutrition recovery was investigated. In amino acid deficiency, L. sphaericus wild strains accumulated polyP, but L. sphaericus ppk mutants did not. The wild strains grew better than ppk mutants in amino acid deficiency medium and, the wild strains regained growth more quickly than ppk mutants in medium with nutrition recovery. Proteomics and bioinformatics analysis revealed that levels of PNP and RNase J protein, the components of RNA degradosome, were up-regulated in L. sphaericus ppk mutants. The regulation of mRNA stability was an important strategy for bacteria to adapt to nutrition deficiency, we suspect that L. sphaericus accumulates polyP to regulate mRNA stability and to adapt to nutritional deficiency and recovery environment.

Key words: Lysinibacillus sphaericus, polyphosphate, amino acids deficiency, environment stresses

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