Preparation of Micropowder of Bacillus methylotrophicus WF-3 and Its Control Effect on Cucumber Anthracnose

doi:10.16409/j.cnki.2095-039x.2018.05.011

Abstract

Abstract: By screening the carriers, wetting agents, dispersing agents and protection agents for Bacillus methylotrophicus WF-3, the optimal formulation were confirmed as follows:the content of filler carbon-white and calcined kaolin, dispersing agent NNO, wetting agent K12 and UV protection agents CMC were 10.0, 85.5%, 2.0%, 2.0% and 0.5%, respectively. The quality detection showed that the spores content of B. methylotrophicus WF-3 micropowder could come up to 2.07×10¹⁰ cfu/g, the pH value was 6.8, the rate of heterozygous bacteria was 2.6%, the water content was 0.98%, the fineness rate was 98% (≤ 8.13 μm), the spore dispersion index was 83.31%, dispersing time was 11.4 s, and thermal decomposition rate of storage was 4.02%, which came up to the formulation standard. Pot experiment demonstrated that the protective effects of B. methylotrophicus WF-3 micropowder on cucumber anthracnose was better than the therapeutic effect, and the efficacies were 77.38% and 72.69%, respectively.

Key words: Bacillus methylotrophicus, micropowder, Colletrichum orbiculare, biocontrol effect

CLC Number:

S476

XIE Xuewen, CHUAI Hongyun, DONG Ruili, YANG Jie, SHI Yanxia, CHAI Ali, LI Baoju. Preparation of Micropowder of Bacillus methylotrophicus WF-3 and Its Control Effect on Cucumber Anthracnose[J]. journal1, 2018, 34(5): 722-728.

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