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journal1 ›› 2018, Vol. 34 ›› Issue (5): 722-728.DOI: 10.16409/j.cnki.2095-039x.2018.05.011

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Preparation of Micropowder of Bacillus methylotrophicus WF-3 and Its Control Effect on Cucumber Anthracnose

XIE Xuewen1, CHUAI Hongyun1, DONG Ruili1, YANG Jie2, SHI Yanxia1, CHAI Ali1, LI Baoju1   

  1. 1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Tibet Autonomous Regional Academy of Agricultural Sciences, Lasa 850000, China
  • Received:2018-03-23 Online:2018-10-08 Published:2018-10-15

Abstract: By screening the carriers, wetting agents, dispersing agents and protection agents for Bacillus methylotrophicus WF-3, the optimal formulation were confirmed as follows:the content of filler carbon-white and calcined kaolin, dispersing agent NNO, wetting agent K12 and UV protection agents CMC were 10.0, 85.5%, 2.0%, 2.0% and 0.5%, respectively. The quality detection showed that the spores content of B. methylotrophicus WF-3 micropowder could come up to 2.07×1010 cfu/g, the pH value was 6.8, the rate of heterozygous bacteria was 2.6%, the water content was 0.98%, the fineness rate was 98% (≤ 8.13 μm), the spore dispersion index was 83.31%, dispersing time was 11.4 s, and thermal decomposition rate of storage was 4.02%, which came up to the formulation standard. Pot experiment demonstrated that the protective effects of B. methylotrophicus WF-3 micropowder on cucumber anthracnose was better than the therapeutic effect, and the efficacies were 77.38% and 72.69%, respectively.

Key words: Bacillus methylotrophicus, micropowder, Colletrichum orbiculare, biocontrol effect

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