Induced Resistance of Crude Protein Produced by Streptomyces roseoflavus Men-myco-93-63 to Cucumber Disease

doi:10.16409/j.cnki.2095-039x.2020.02.013

Abstract

Abstract: Strempomyces roseoflavus Men-myco-93-63 is an antagonistic Streptomyces sp. isolated from the soil where potato scab could decline naturally. The strain and its metabolites have strong inhibitory effects on many important plant pathogens. In order to investigate the induction resistance of the crude protein produced by Men-myco-93-63 to cucumber disease, leaf inoculation was used in vitro. The results showed that the lesion diameter of gray mold in the leaves of cucumber induced by crude protein was significantly smaller than that of buffer solution. Detected by tissue staining and ultraviolet spectrophotometry, the accumulation of reactive oxygen species (ROS) and activity of resistance-related enzymes such as superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO), were significantly increased in cucumber leaves induced by crude protein compared with buffer solution. Quantitative Real-time PCR (qRT-PCR) showed that the crude protein could also induce the up-regulation of the expression of resistance-related genes such as PR-1a, PR-3, PR-9 and NPR1 in cucumber leaves, indicating that the crude protein produced by Men-myco-93-63 could induce disease resistance in cucumber.

Key words: Streptomyces roseoflavus, protein elicitor, induced disease resistance, defense enzyme activity, resistance-related genes

CLC Number:

S476

GUO Jinghong, HAN Xing, ZHEN Danmei, MENG Qingfang, KANG Zhanhai, WANG Bing, LI Yaning, LIU Daqun. Induced Resistance of Crude Protein Produced by Streptomyces roseoflavus Men-myco-93-63 to Cucumber Disease[J]. Chinese Journal Of Biological Control, 2020, 36(2): 272-279.

References

[1] 张易. 灰葡萄孢蛋白激发子BcSpl1诱导番茄抗病机制研究[D]. 北京:中国农业科学院, 2015.
[2] 刘孟洁. 稻瘟菌蛋白激发子MoHrip2结构与功能及互作蛋白研究[D]. 北京:中国农业科学院, 2016.
[3] Lamb C, Dixon R A. The oxidative burst in plant disease resistance[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1997, 48(1):251-275.
[4] 闫艳华, 王海宽, 肖瑞峰, 等. 一株乳酸菌对番茄灰霉病的防效及对几种防御酶活性的影响[J]. 微生物学通报, 2011, 38(12):1801-1806.
[5] 田华. 烟草炭疽菌Colletotrichum destructivum诱抗蛋白及其作用分析[D]. 北京:中国农业科学院, 2016.
[6] 邱德文. 我国植物免疫诱导技术的研究现状与趋势分析[J]. 植物保护, 2016, 42(5):10-14.
[7] Liu D Q. Biological control of Streptomyces scabies and other plant pathogens[D]. USA:The University of Minnesota, 1992.
[8] 冯龙, 魏娇洋, 陈英化, 等. 玫瑰黄链霉菌Men-myco-93-63营养型片剂的研制及应用[J]. 河北农业大学学报, 2014, 37(5):77-81.
[9] 赤国彤, 李亚宁, 孟庆芳, 等. Men-myco-93-63生物制剂防治瓜类白粉病药效试验[J]. 湖南农业科学, 2011(23):89-91.
[10] 张艳杰, 魏学军, 寇宏达, 等. 玫瑰黄链霉菌Men-myco-93-63抗南方根结线虫相关酶活性及其防效[J]. 西北农业学报, 2013, 22(4):184-190.
[11] 郭景红, 甄丹妹, 韩兴, 等. 玫瑰黄链霉菌Men-myco-93-63胞外分泌蛋白对黄瓜白粉病的诱导抗性初报[J]. 中国植保导刊, 2018, 38(5):5-9.
[12] 张艳. 生防菌株Men-myco-93-63发酵工艺优化研究[D]. 保定:河北农业大学, 2006.
[13] Marcos F, González C, Brito N. BcSpl1, a cerato-platanin family protein, contributes to Botrytis cinerea virulence and elicits the hypersensitive response in the host[J]. New Phytologist, 2011, 2(192):483-495.
[14] Alizadeh H, Behboudi K, Ahmadzadeh M, et al. Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp. Ps14.[J]. Biological Control, 2013, 65(1):14-23.
[15] Shoresh M, Yedidia I, Chet I. Involvement of jasmonic acid/Ethylene signaling pathway in the systemic resistance induced in cucumber by Trichoderma asperellum T203[J]. Phytopathology, 2005, 95(1):76-84.
[16] Livak K J, Schmittgen T D. Anailsis of relative gene expression data using real time quantitative PCR and method[J]. Methods, 2001, 25:402-408.
[17] 朱红英. 控释肥料在玉米上的应用效应研究[D]. 青岛:山东农业大学, 2004.
[18] 王宁波. 解淀粉芽胞杆菌NC6蛋白激发子PeBA1的分离鉴定及功能研究[D]. 北京:中国农业科学院, 2016.
[19] 王颢潜. 侧孢短芽胞杆菌A60激发子PeBL1鉴定和基因功能的研究[D]. 北京:中国农业科学院, 2015.
[20] 王炳楠, 王双超, 檀贝贝, 等. 大丽轮枝菌蛋白激发子PevD1诱导的烟草对烟草花叶病毒(TMV)系统获得性抗性及其分子机制[J]. 农业生物技术学报, 2012, 20(2):188-195.