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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (5): 771-777.DOI: 10.16409/j.cnki.2095-039x.2020.05.021

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Study on Mutagenesis of Paenibacillus polymyxa LB-9 and Its Effect of Bacteriostasis and Disease Prevention

LI Xiaojie, LIU Chang, LI Chengjun, YAO Chenxiao, BAI Jingke, YU Bingna, QIU Rui, CHEN Yuguo, LI Shujun   

  1. Key Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area/Tobacco Research Institute of Henan Academy of Agricultural Sciences, Xuchang 461000, China
  • Received:2020-05-07 Online:2020-10-08 Published:2020-11-20

Abstract: In order to obtain the antagonistic strain with stronger inhibition and better biocontrol effect on Phytophthora tabacum, gene mutation of Paenibacillus polymyxa LB-9 was carried out. The Tn-5 insertion mutant library of LB-9 strain was constructed by electrotrans formation using protoplast, and about 2000 transformants were obtained. Three mutants were screened out through plate confrontation culture. Among them, the inhibition effect of A13 mutant on P. tabacum was over 80%, which was higher than that of LB-9 more than 20%. The control effect of LB-9 and its positive mutant strain A13 on tobacco black shank was studied in greenhouse and the expression of resistance related genes were analyzed by real-time fluorescence quantitative PCR. The results showed that LB-9 and its positive mutant A13 had good control effect on tobacco black shank in 7 days after treatment. The control effect of A13 on tobacco black shank was higher than that of LB-9, which the control effect was more than 80%. At the same time, it could induce the up-regulation expression of salicylic acid signal pathway marker gene PR1a, ethylene signal pathway response gene PR-3b and jasmonic acid signal pathway regulator gene COI1 in tobacco plants. This study provides theoretical support for the study of breeding methods of P. polymyxa by insertional mutation, and is of great significance for biological control of tobacco black shank.

Key words: Paenibacillus polymyxa, mutagenesis, Phytophthora tabacum, control effect, induced disease resistance

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