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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (5): 795-801.DOI: 10.16409/j.cnki.2095-039x.2020.05.015

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Visual Detection of Entomogenous Fungi Isaria fumosorosea by Loop-mediated Isothermal Amplification Technology

LIU Xiaofei1, CHEN Ruiyuan2, ZHENG Yu1   

  1. 1. Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
    2. Fuzhou No.1 High School, Fuzhou 350000, China
  • Received:2019-12-02 Online:2020-10-08 Published:2020-11-20

Abstract: Isaria fumosorosea is an important entomogenous fungus and has become an important resource for microbial pesticides. Due to the lack of effective methods, a large number of rapid and accurate detections of I. fumosorosea in the diseased pests are impossible, which limits the scientific evaluation of the control effect of related biocontrol strains. In this study, through the primers designed and system optimization of the loop-mediated isothermal amplification (LAMP), a visual LAMP detection method was established for I. fumosorosea. The detection method had strong specificity, high accuracy, and no cross reaction with other common biocontrol strains and pathogenic bacteria; the detection time was short, and a positive result could be obtained in 60 minutes; the sensitivity was high, which was 100 fold that of conventional PCR detection methods. The establishment of this method provides a new technology for the virulence determination and safety evaluation of I. fumosorosea under field conditions.

Key words: entomogenous fungi, Isaria fumosorosea, LAMP, visual detection

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