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Chinese Journal of Biological Control ›› 2020, Vol. 36 ›› Issue (6): 920-928.DOI: 10.16409/j.cnki.2095-039x.2020.06.023

• RESEARCH REPORTS • Previous Articles    

Studies on the Expression and Silencing Efficiency of Aphis glycines Trehalose-6-Phosphate Synthase at Different Temperatures

YANG Hongjia1, WANG Xiaoyun1, JIN Myong Cha1,2, FAN Dong1   

  1. 1. College of Agronomy, Northeast Agricultural University, Harbin 150030, China;
    2. Kyeungsang Sariwon Agricultural University, Pyong Yang 95003, DPR of Korea
  • Received:2020-09-27 Published:2021-01-09

Abstract: Trehalose is the most important carbohydrate in insect hemolymph, and trehalose-6-phosphate synthase (TPS) is a key enzyme in the trehalose synthesis pathway in insects. In this study, a cDNA sequence of soybean aphid Aphis glycines (Matsumura) trehalose synthase was obtained through transcriptome sequencing, and named AgTPS (GenBank accession number: MN068811). The cDNA sequence is 4174 bp in length, contains an open reading frame of 2559 bp and encodes 852 amino acids. The isoelectric point of the deduced amino acid is 5.98. The molecular mass is 95.8 kDa. The deduced amino acid has a typical TPS domain. AgTPS was inhibited at different temperatures. It was at 5 ℃ that AgTPS silenced for a long duration. When inhibited at 30 ℃ for 6 h, the gene had the best silence effect and the inhibition rate was 79.48%. The survival and reproduction of soybean aphid were also most affected at 30 ℃. The results indicated that AgTPS was involved in the stress resistance of soybean aphid. This study provided theoretical basis for the prevention and control of soybean aphid by RNA interference technology.

Key words: Aphis glycines, trehalose-6-phosphate synthase, temperature stress, RNA interference

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