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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (2): 286-296.DOI: 10.16409/j.cnki.2095-039x.2021.02.010

• RESEARCH REPORTS • Previous Articles    

Effect of Aureobasidium pullulans PA-2 Metabolism of Chenopodium album Leaves

GAO Hanfeng, YANG Ying, CHEN Hongyu, CHENG Liang, GUO Qingyun   

  1. Academy of Agriculture and Forestry Sciences, Qinghai University/Key Laboratory of Integrated Management of Agricultural Pests in Qinghai Province/Xining Crop Pest Scientific Observation and Experimental Station, Ministry of Agriculture and Rural Affairs, Xining 810016, China
  • Received:2020-08-10 Published:2021-04-14

Abstract: To clarify the effect of Aureobasidium pullulans PA-2 infection on metabolism of Chenopodium album leaves, the metabolomics method based on liquid chromatography-mass spectrometry (LC-MS) was used to analyze the differential metabolites in C. album leaves infected by the strain PA-2. Through orthogonal partial least squares discriminant analysis (OPLS-DA), a total of 187 metabolites with significant differences (VIP>1 and P<0.05) were screened 24 h after the treatment of PA-2. Among them, 148 were up-regulated and 37 were down-regulated. Adenosine and betaine can be used as the main biomarkers after 24 h. KEGG enrichment pathway analysis indicated that these differential metabolites were mainly enriched in 3 metabolic pathways, including global and overview maps, amino acid metabolism and carbohydrate metabolism. The obvious changes in purine metabolism, biosynthesis of secondary metabolites, amino acid metabolism and carbohydrate metabolism in C. album leaves may participate in the PA-2 infection to C. album. The above results provide a theoretical basis for further research on the biocontrol mechanism of PA-2 infecting C. album.

Key words: Aureobasidium pullulans PA-2, Chenopodium album, metabolomics, differential metabolites, metabolic pathway

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