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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (4): 817-825.DOI: 10.16409/j.cnki.2095-039x.2021.04.009

• RESEARCH REPORTS • Previous Articles     Next Articles

Isolation, Identification, and Biological Characteristics of the Biocontrol Fungi NJC-16 for Eleusine indica

GU Qiongnan1, OU Xiang2, CHU Shihai1, HUANG Qichao1, CHEN Anan1, LI Ruhai1   

  1. 1. Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Crop Diseases, Insect Pests and Weeds Control/Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;
    2. Guilin Customs of the People's Republic of China, Guilin 541004, China
  • Received:2020-11-13 Published:2021-09-18

Abstract: In order to screen and obtain a low toxicity and efficient bioherbicide to goosegrass, strain NJC-16, a pathogenic fungus of anthracnose disease of goosegrass in Hubei Province, was obtained by tissue isolation. The strain was identified as Colletotrichum eleusines by morphological characterization combined with ITS sequence analysis. The optimum growth temperature for the strain was 25℃. No significant differences were found in the mycelial growth between different illuminations. The optimal carbon source for mycelial growth was starch, and the optimal nitrogen source for mycelial growth was yeast. The optimum pH value for mycelial growth was 6~9. Hyphal viability completely lost when hyphal agar was treated at 65℃ for 10 min. Indoor test showed that disease incidence of goosegrass reached 83.2% 21 d after inoculation of NJC-16 spore suspension, and the inhibition rate of goosegrass fresh weight was 73%. However, the strain was not effective to Echinochloa crusgalli, Digitaria sanguinalis, Leptochloa chinesis, Vicia sativa and Amaranthus retroflexus. Crop safety tests showed that the spore suspension was safe to maize, wheat and rice crops. Taken together, strain NJC-16 has the potential to be developed as a mycoherbicide.

Key words: goosegrass, Colletotrichum eleusines, biological characteristics, mycoherbicide

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