Screening, Identification and Biocontrol Mechanism of Antagonistic Bacteria against Root Rot of Salvia miltiorrhiza

doi:10.16409/j.cnki.2095-039x.2021.04.011

Abstract

Abstract: Root rot of Salvia miltiorrhiza caused by Fusarium solani is one of the main soil borne diseases of Salvia miltiorrhiza. In this study, an endophytic antagonistic strain DS-R5 was isolated from Salvia miltiorrhiza by dilution plating method, and identified to be Paenibacillus polymyxa by morphology and molecular biology identification. The inhibition mechanism of DS-R5 against F. solani was investigated by spore germination test, optical microscope, scanning electron microscope (SEM) and transmission electron microscope (TEM) observation. The results showed that the strain DS-R5 had obvious inhibitory effect on the spore germination of F. solani. Optical microscope observation revealed that the hyphae inhibited by the strain DS-R5 was distorted, twisted and broken, and local fracture appeared in some parts. SEM observation showed that the hyphae were broken in large quantity and there was some hyphal debris. The hyphae thickness was different, the surface was rough, and there was leakage of intracellular substances. TEM observation revealed that the hyphae cells were abnormal, no complete organelles existed, cytoplasm flowed out and abnormal cavities appeared in the hyphae. Pot experiments showed that the control efficacy of the strain DS-R5 on root rot of Salvia miltiorrhiza was 61.4%. In conclusion, the strain DS-R5 has good biocontrol potential against root rot of Salvia miltiorrhiza and has the prospect of further development.

Key words: Salvia miltiorrhiza, endophytic antagonistic bacteria, screening and identification, inhibition mechanism, pot experiment

CLC Number:

S476

XU Le, WANG Ziqiang, ZHANG Shuang, XING Qian, LIU Lina, SA Rongbo. Screening, Identification and Biocontrol Mechanism of Antagonistic Bacteria against Root Rot of Salvia miltiorrhiza[J]. Chinese Journal of Biological Control, 2021, 37(4): 846-854.

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