Effects of Aspergillus flavipes ASD on the Structure of Rhizosphere Fungi and Function of Soil infected by Phytophthora capsici in Pepper

doi:10.16409/j.cnki.2095-039x.2021.04.015

Abstract

Abstract: To explain the soil microbial regulation mechanism of biocontrol strain Aspergillus flavipes ASD against pepper blight, the effects of rhizosphere soil characteristics and fungal diversity of affected pepper were analyzed by chemical methods and Illumina HiSeq platform after application of ASD strain solution. The results showed that the application of ASD strain solution could significantly increase the contents of nitrate nitrogen and available potassium in the affected soil, while decreased the content of ammonium nitrogen and the activity of N-acetyl-β-D-aminoglycosidase. The application of ASD bacterial fluid increased the diversity and abundance of fungi in the rhizosphere soil of the affected pepper, which resulted in the variation of dominant fungal community. Correlation analysis of dominant fungi and disease incidence showed that 9 genuses such as Mycothermus and Myceliophthora with an increasing abundance were negatively correlated with the disease incidence, whereas Clitopilus and Parasola with a decreasing abundance were positively correlated with the incidence. Correlation analysis with environmental factors showed that the contents of available potassium and nitrate nitrogen were significantly positively correlated with fungal diversity after ASD treatment, and positively affected 10 genuses such as Conocybe. However, the content of ammonium nitrogen and the activity of N-acetyl-β-D-aminoglycosidase activity were opposite correlation. In summary, Aspergillus flavipes ASD could increase the rhizosphere soil fungal diversity of pepper blight, change the soil nutritional status and dominant fungal community structure, thereby reduce the incidence of pepper blight. These results could provide the theoretical basis for the application of ASD strain in production practice.

Key words: Aspergillus flavipes, pepper blight, Fungal diversity, soil nutrient, soil enzyme activity

CLC Number:

S476

WANG Hui, LIU Li, HUANG Yufei, ZOU Chunlei, ZHAO Ying, WANG Qi, LIU Changyuan. Effects of Aspergillus flavipes ASD on the Structure of Rhizosphere Fungi and Function of Soil infected by Phytophthora capsici in Pepper[J]. Chinese Journal of Biological Control, 2021, 37(4): 796-803.

References

[1] 冯东昕, 李宝栋. 辣椒疫病病原菌及抗病育种研究进展[J]. 中国蔬菜, 1999(2):50-54.
[2] 肖淑芹, 陆秀华, 刘惕若. 黑龙江省辣椒疫病发生情况调查[J]. 黑龙江农业科学, 2002(5):45-46.
[3] 秦维彩, 闫晓静, 檀根甲, 等. 烯酰吗啉与嘧菌酯对辣椒疫霉病菌生物活性的比较[J]. 植物保护学报, 2010, 37(1):42-48.
[4] Gisi U, Cohen Y. Resistance to phenylamide fungicides:A case study with Phytophthora infestans involving mating type and race structure[J]. Annual Review of Phytopathology, 1996, 34:549-572.
[5] 王辉, 刘长远, 赵奎华, 等. 辽宁省辣椒疫病菌多态性及致病力分化研究初探[J]. 微生物学通报, 2012, 39(2):180-190.
[6] Liu M X, Yi Z L, Zhao Y L, et al. Research advances of biological control on turf grass disease[J]. Acta Prataculturae Sinica, 2004, 13(6):1-7.
[7] 李兴龙, 李彦忠. 土传病害生物防治研究进展[J]. 草业学报, 2015, 24(3):204-212.
[8] Katan J. Diseases caused by soilborne pathogens:biology, management and challenges[J]. Journal of Plant Pathology, 2017, 99(2):305-315.
[9] 侯劭炜. 辣椒疫病防治中AM真菌的作用及应用研究[D]. 杨凌:西北农林科技大学, 2019.
[10] 王光飞, 马艳, 郭德杰, 等. 生物质炭介导生防微生物抑制辣椒疫霉的作用[J]. 中国生态农业学报, 2019, 27(7):1015-1023.
[11] 李祝, 丛铭, 刘松, 等. 黑曲霉抗菌活性组分分离研究[J]. 食品安全质量检测学报, 2014(12):3982-3987.
[12] Li X J, Zhang Q, Zhang A L, et al. Metabolites from Aspergillus fumigatus, an endophytic fungus associated with Melia azedarach, and their antifungal, antifeedant, and toxic activities[J]. Journal of Agricultural and Food Chemistry, 2012, 60(13):3424-3431.
[13] 王辉, 毕于慧, 于志国, 等. 黄柄曲霉ASD次生代谢产物的鉴定及抑菌活性[J]. 农药学学报, 2020(3):454-460.
[14] 李淑彬, 王军, 杨劲松, 等. 抗真菌抗生素179M产生菌的分离鉴定和生理特性研究[J]. 菌物系统, 2001(3):362-367.
[15] 徐丽慧, 曾蓉, 高士刚, 等. 土壤真菌多样性对土传病害影响的研究进展[J]. 上海农业学报, 2017, 33(3):161-165.
[16] Rime D, Nazaret S, Gourbiere F, et al. Comparison of sandy soils suppressive or conducive to ectoparasitic nematode damage on sugarcane[J]. Ecology and Population Biology, 2003, 93(11):1437-1444.
[17] Perez P A, Edel H V, Alabouvette C, et al. Response of soil microbial communities to compost amendments[J]. Soil Biology& Biochemistry, 2006, 38(3):460-470.
[18] 辛景树, 田有国, 任意, 等. NY/T 1121.1-2006, 土壤检测第1部分:土壤样品的采集、处理和贮存[S]. 北京:中国农业出版社, 2006.
[19] 董艳, 杨智仙, 董坤, 等. 施氮水平对蚕豆枯萎病和根际微生物代谢功能多样性的影响[J]. 应用生态学报, 2013, 24(4):1101-1108.
[20] 方中达. 植病研究方法第三版[M]. 北京:中国农业出版社, 1998. 12, 41.
[21] 任意, 辛景树, 田有国, 等. NY/T 1121.6-2006土壤检测第6部分:土壤有机质的测定[S]. 北京:中国农业出版社, 2006.
[22] 段铁城, 高祥照, 贾玮, 等. NY/T 1848-2010中性、石灰性土壤铵态氮、有效磷、速效钾的测定联合浸提-比色法[S]. 北京:中国农业出版社, 2010.
[23] 孙波, 宋歌, 张玉铭, 等. GB/T 32737-2016土壤硝态氮的测定紫外分光光度法[S]. 北京, 中国标准出版社, 2016.
[24] 辛景树, 郑磊, 钟杭, 等. NY/T 1121.7-2014土壤检测第7部分:土壤有效磷的测定[S]. 北京:中国农业出版社, 2014.
[25] Hanan G, Diab E A, Vivian V, et al. The use of phospholipids fatty acids (PLFA) in the determination of rhizosphere specific microbial communities (RSMC) of two wheat cultivars[J]. Plant and Soil, 2001, 228:291-297.
[26] Ladd J N, Butler J H A. Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates[J]. Soil Biology and Biochemistry, 1972, 4(1):19-30.
[27] Lowry O H, Rosebrough N J, Farr A L, et al. Protein measurement with the Folin phenol reagent[J]. Journal of Biological Chemistry, 1951, 193(1):256-275.
[28] Pind A, Freeman C, Lock M A. Enzymatic degradation of phenolic materials in peatlands-measurement of phenol oxidase activity[J]. Plant Soil, 1994, 159:227-231.
[29] Weaver R W, Angle S, Bottomley P, et al. Methods of soil analysis. Part II:Microbiological and biochemical properties[J]. Soil Science Society of America, 1994(22):775-833.
[30] Richard P D. Methods of Soil Enzymology[M]. Madison:American Society of Agronomy, 2011, 45-64.
[31] Parham J A, Deng S P. Detection, quantify cation and characterization of β-glucosaminidase activity in soil[J]. Soil Biology and Biochemistry, 2000, 32:1183-1190.
[32] Barbi F, Prudent E, Vallon L, et al. Tree species select diverse soil fungal communities expressing different sets of lignocellulolytic enzyme-encoding genes[J]. Soil Biology and Biochemistry, 2016, 100:149-159.
[33] Smith K P, Goodman R M. Host variation for interactions with beneficial plant-associated microbes[J]. Annual Review of Phytopathology, 1999, 37:473-491.
[34] 杨丽娟, 张玉龙. 保护地菜田土壤硝酸盐积累及其调控措施的研究进展[J]. 土壤通报, 2001, 32(2):66-69.
[35] 李凤霞, 王长军, 刘丽丹, 等. 土地利用方式对宁夏平原土壤氮素含量及其硝化作用的影响[J]. 河南农业科学, 2019, 48(2):77-82.
[36] 郭媛, 李宜联, 郭策, 等. 不同氮素添加对不同土地利用方式黑土氮素转化特征的影响[J]. 水土保持学报, 2021, 35(1):236-243.
[37] Olander L P, Vitousek P M. Regulation of soil phosphatase and chitinase activity by N and P availability[J]. Biogeochemistry, 2000, 49(2):175-191.
[38] 王冰冰, 曲来叶, 马克明, 等. 岷江上游干旱河谷优势灌丛群落土壤生态酶化学计量特征[J]. 生态学报, 2015, 35(18):6078-6088.
[39] 刘国坤, 张络升, 潘东明. 淡紫拟青霉PL050705菌株的根际定殖及对根际真菌群落结构影响[J]. 中国农学通报, 2009, 25(18):324-328.
[40] 扈进冬, 吴远征, 魏艳丽, 等. 木霉拌种剂对小麦根际土壤真菌群落多样性的影响[J]. 山东科学, 2019, 31(2):46-51.
[41] 张文浩, 门梦琪, 许本姝, 等. 牛粪稻秸新型静态堆肥中真菌群落组成的动态特征[J]. 农业环境科学学报, 2018, 37(9):2029-2036.
[42] 杨帆. 转录因子CRE1及ACE1在嗜热毁丝霉纤维素酶基因表达调控中的作用[D]. 深圳:深圳大学, 2015.
[43] 曾思泉, 凌娟, 林丽云, 等. 1株红树林来源枝孢属真菌的分离鉴定及纤维素酶性质分析[J]. 微生物学杂志, 2018, 38(2):37-42.
[44] 宋健, 张海剑, 刘莉, 等. 高通量测序分析高温覆膜对韭菜根际微生物多样性的影响[J]. 中国生物防治学报, 2000, 36(6):938-945.
[45] 何苑皞,周国英,王圣洁, 等. 杉木人工林土壤真菌遗传多样性[J]. 生态学报, 2014, 34(10):2725-2736.