Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (4): 796-803.DOI: 10.16409/j.cnki.2095-039x.2021.04.015

• RESEARCH REPORTS • Previous Articles     Next Articles

Effects of Aspergillus flavipes ASD on the Structure of Rhizosphere Fungi and Function of Soil infected by Phytophthora capsici in Pepper

WANG Hui1, LIU Li1, HUANG Yufei2, ZOU Chunlei3, ZHAO Ying4, WANG Qi5, LIU Changyuan1   

  1. 1. Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;
    2. College of Plant Protection, Shenyang Agricultural University, Shenyang 100866, China;
    3. Vegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;
    4. Plant Nutrition and Environmental Resources Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;
    5. College of Plant Protection, China Agricultural University, Beijing 100193, China
  • Received:2020-10-26 Published:2021-09-18

Abstract: To explain the soil microbial regulation mechanism of biocontrol strain Aspergillus flavipes ASD against pepper blight, the effects of rhizosphere soil characteristics and fungal diversity of affected pepper were analyzed by chemical methods and Illumina HiSeq platform after application of ASD strain solution. The results showed that the application of ASD strain solution could significantly increase the contents of nitrate nitrogen and available potassium in the affected soil, while decreased the content of ammonium nitrogen and the activity of N-acetyl-β-D-aminoglycosidase. The application of ASD bacterial fluid increased the diversity and abundance of fungi in the rhizosphere soil of the affected pepper, which resulted in the variation of dominant fungal community. Correlation analysis of dominant fungi and disease incidence showed that 9 genuses such as Mycothermus and Myceliophthora with an increasing abundance were negatively correlated with the disease incidence, whereas Clitopilus and Parasola with a decreasing abundance were positively correlated with the incidence. Correlation analysis with environmental factors showed that the contents of available potassium and nitrate nitrogen were significantly positively correlated with fungal diversity after ASD treatment, and positively affected 10 genuses such as Conocybe. However, the content of ammonium nitrogen and the activity of N-acetyl-β-D-aminoglycosidase activity were opposite correlation. In summary, Aspergillus flavipes ASD could increase the rhizosphere soil fungal diversity of pepper blight, change the soil nutritional status and dominant fungal community structure, thereby reduce the incidence of pepper blight. These results could provide the theoretical basis for the application of ASD strain in production practice.

Key words: Aspergillus flavipes, pepper blight, Fungal diversity, soil nutrient, soil enzyme activity

CLC Number: