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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 205-214.DOI: 10.16409/j.cnki.2095-039x.2021.05.005

• RESEARCH REPORTS • Previous Articles     Next Articles

The Relationship between Symbiotic Bacteria and Wing Dymorphism in Rhopalosiphum padi

XU Chao, WANG Lisha, ZHU Xiangzhen, WANG Li, LI Dongyang, ZHANG Kaixin, JI Jichao, LUO Junyu   

  1. State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
  • Received:2021-01-29 Online:2022-02-08 Published:2022-03-01

Abstract: The purpose of this study was to explore the relationship between symbiotic bacteria and the wing dimorphism in Rhopalosiphum padi (Linnaeus) and to enrich the understanding of the mechanism of insect phenotypic plasticity. This study used Illumina MiSeq high-throughput sequencing platform to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the alate and apterous morphs of R. padi, respectively. One hundred and seventy-four operational taxonomic units (OTUs) belonging to 10 phyla, 18 classes, 42 orders, 66 families and 98 genera were identified from all samples. At the genus level, Buchnera, as the dominant bacteria, had the highest abundance (83.21%), followed by Staphylococcus (5.25%) and Arthrobacter (2.08%). The diversity of symbionts varied between the two morphs. Apterous morph had more bacterial diversity (174 OTUs belonging to 10 phyla, 18 classes, and 66 families) than alate morphs (151 OTUs belonging to 9 phyla, 14 classes, and56 families). The abundance of various OTUs were significantly different between these two morphs. Among these OTUs, only Buchnera represented a high proportion (95.57%) in alate morphs but were present in low abundance (70.84%) in apterous morphs. The survey showed that there were differences in the diversity and abundance of symbionts between winged and wingless R. padi. These results are helpful to further understand the microorganisms that may be involved in insect wing dimorphism, and contribute to control the dispersal of this pest by reinfection of symbionts or targeting symbiosis-related host genes by RNA interference in future.

Key words: Rhopalosiphum padi, symbionts, wing polyphenism, 16S rDNA sequencing

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