Screening of Three Strains for Degrading Flurochloridone and Their Biodegradation Effects

doi:10.16409/j.cnki.2095-039x.2021.06.025

Abstract

Abstract: To explore the strains with the ability to degrade flurochloridone in the soil. Enrichment methods and plate dilution methods were used for screening strains, and the degradation rate of each strain for flurochloridone was detected by Liquid Chromatography-Tandem Mass Spectrometry. The strains with dominant degradation were identified by combined with morphological observations, 16S rDNA and ITS sequencing analysis, and the safety evaluation of each strain was determined with laboratory bioassays. The results showed that three strains FL-M-2, FL-M-3, FL-G-2 with a significant degradation rate from soil were identified as Purpureocillium lavendrulum, Didymellaceae sp. and Streptomyces parvus, respectively. Three strains cultured with 180 r/min, inoculation amount 5%, culture temperature at 28 ℃ for 7 days in culture medium have the degradation rate of 39.63%, 72.09% and 68.80% for the concentration of 1 mg/L flurochloridone, respectively. Among them, the strain FL-G-2 has the best degradation effect, and its degradation rate reached 84.37% after 14 days of culture. The fermentation broth of three strains had no obvious inhibitory effect on the growth of wheat and rape seedlings. This research provides a basis for analysis of the mechanism of microorganism degradation of flurochloridone, and also offers new strains resource for further development of the degradation of pyrrolidone herbicides.

Key words: flurochloridone, microbial degradation, degradation effect, safety

CLC Number:

S476

LEI Xiangrong, SHEN Shuo, LI Wei. Screening of Three Strains for Degrading Flurochloridone and Their Biodegradation Effects[J]. Chinese Journal of Biological Control, 2021, 37(6): 1231-1240.

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