Antifungal and Field-Control Effects of Eugenol and Carvacrol on Ustilaginoidea virens

doi:10.16409/j.cnki.2095-039x.2023.02.061

Abstract

Abstract: To develop new fungicides based on natural components for the prevention and control of rice false smut, eugenol and carvacrol, two natural plant-derived terpenoids with rich bioactivities and wide sources, were investigated in this study. Concentration gradient tests showed that both of these two compounds could significantly inhibit the mycelial growth and spore germination of Ustilaginoidea virens in a concentration- dependent manner. At a concentration of 0.2 mmol/L, the inhibition rates of eugenol and carvacrol on mycelial growth were 12.53% and 44.39%, respectively, and the inhibition rates on spore germination were 94.02% and 72.24%, respectively. The scanning electron microscopy confirmed that both eugenol and carvacrol caused disruptions in the integrity of U. virens hyphae. The field efficacy experiments were further performed and the results showed that both of these two natural products were effective in inhibiting the incidence of rice false smut and reducing the accumulation of ustiloxin A in paddy. In conclusion, eugenol and carvacrol were successfully applied to control rice false smut and prevent the production of ustiloxin A in this study, and the underlying mechanisms were preliminarily analyzed, providing their broad application prospects in the future.

Key words: eugenol, carvacrol, Ustilaginoidea virens, rice false smut, ustiloxin a, prevention and control

CLC Number:

S476

HU Zheng, FAN Kai, YU Yinan, YOU Jiaqi, WANG Xinyi, GUO Dakai, HUANG Qingwen, MENG Jiajia, GUO Wenbo, NIE Dongxia, HAN Zheng. Antifungal and Field-Control Effects of Eugenol and Carvacrol on Ustilaginoidea virens[J]. Chinese Journal of Biological Control, 2023, 39(6): 1446-1454.

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