Joint Control of Sesame Bacterial Wilt with Lime and Zhongshengmycin

doi:10.16409/j.cnki.2095-039x.2020.03.026

Abstract

Abstract: To explore the effective prevention and control of Ralatonia solanacearumin high-density cultivation, the relationship between the pH distribution of soil and the occurrence of bacterial wilt of sesame were analyzed in Jiangxi province, the safety of quicklime soil treatment to sesame and its resistance to bacterial wilt were assessed, and a trail was carried out on the prevention and control of bacterial wilt of sesame by the combination of Zhongshengmycin spray and quicklime soil treatment. The results showed that the pH value of soil samples in the field where severe symptoms of bacterial wilt observed in sesame was concentrated in the range of 4.5-5.5, and the soil samples with pH value of 4.5-5.5 accounted for 69.17% in sesame fields.Withquicklime1125-2250 kg/hm² soil treatment, the seedling emergence rates of Ganzhi 5 and Yuzhi 11 were not significant different from that of the blank control group. The seedling qualities of these two varieties were better than the blank control, especially root-collar diameters of these two varieties were significantly higher than the blank control. The disease index from the budding stage to the mature stage was always lower than the blank control, and the disease index from the bud stage to the initial flower stage decreased by more than 30% compared with the blank control. The soil was treated with quick lime (1125-2250 kg/hm²) before sowing, and the sesame seedlings were sprayed once for each time at a dosage of 3% Zhongshengmycin wetable power of 22.5 g.ai/hm² at the seedling stage and the initial flowering stage. The combined control effect on bacterial wilt were 73.46%-74.89%, which was significantly higher than the control effect of 3% Zhongshengmycinalone for 3 times (P<0.05). With the combination of 3% Zhongshengmycin spray and quicklime soil treatment, the yields of sesame increased 31.33%-32.49%, which was better than that of 3% Zhongshengmycinalone.

Key words: sesame, Ralstonia solanacearum, lime, Zhongshengmycin, prevention and control

CLC Number:

S432.1

LI Xinshen, CHEN Jian, XIAO Yunping, HUANG Ruirong, WEI Lingen, HUA Juling. Joint Control of Sesame Bacterial Wilt with Lime and Zhongshengmycin[J]. Chinese Journal Of Biological Control, 2020, 36(3): 472-478.

References

[1] 华菊玲, 胡白石, 李湘民, 等. 芝麻细菌性青枯病病原菌及其生化变种鉴定[J]. 植物保护学报, 2012, 39(1):39-44.
[2] 徐进, 冯洁. 植物青枯菌遗传多样性及致病基因组学研究进展[J]. 中国农业科学, 2013, 46(14):2902-2909.
[3] Li X S, Huang X M, Chen G Y, et al. Complete genome sequence of the sesame pathogen Ralstonia solanacearum strain SEPPX 05[J].Genes and Genomics, 2018, 40(6):657-668.
[4] Salanoubat M, Genin S, Artiguenave F, et al. Genome sequence of the plant pathogen Ralstonia solanacearum[J]. Nature, 2002, 415(6871):497-502.
[5] Wicker E, Grassart L, Coranson-Beaudu R, et al. Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential[J]. Applied and Environmental Microbiology, 2007, 71(21):6790-6801.
[6] 付丽军, 王永存, 王向东, 等. 6种杀菌剂对姜青枯病的防治效果[J]. 植物保护, 2019, 45(1):216-220.
[7] 赵华, 吴珉, 何红梅, 等. 高效液相色谱法测定噻唑锌在水、土壤及黄瓜中的残留[J]. 农药学学报, 2017, 19(2):217-222.
[8] 张忠启, 茆彭, 于东升, 等. 近25年来典型红壤区土壤pH变化特征——以江西省余江县为例[J]. 土壤学报, 2018, 55(6):1545-1553.
[9] Wei Z, Gu Y, Friman V P, et al. Initial soil microbiome composition and functioning predetermine future plant health[J]. Science Advances, 2019, 5(9):eaaw0759.
[10] 郑世燕, 陈弟军, 丁伟, 等. 烟草青枯病发病烟株根际土壤营养分析[J]. 中国烟草学报, 2014, 20(4):57-64.
[11] 郑雪芳, 刘波, 朱育菁, 等. 设施番茄连作障碍土壤修复及其对青枯病害的防治效果[J]. 中国生物防治学报, 2018, 34(1):117-123.
[12] 伍朝荣, 黄飞, 高阳, 等. 土壤生物消毒对番茄青枯病的防控、土壤理化特性和微生物群落的影响[J]. 生态学杂志, 2017, 36(7):1933-1940.
[13] Sharma J P, Kumar S. Management of Ralstonia wilt through soil disinfectant, mulch, lime and cakes in tomato (Lycopersiconesculentum)[J]. Indian Journal of Agricultural Sciences, 2013, 70(1):17-19.
[14] Overbeek L V,Runia W, Kastelein P, et al. Anaerobic disinfestation of tare soils contaminated with Ralstonia solanacearum biovar 2 and Globodera pallida[J]. European Journal of Plant Pathology, 2014, 138(2):323-330.
[15] 杨佩文, 杨群辉, 倪明, 等. 烟草青枯病发流行气象因素分析及土壤调理防控技术[J]. 西南农业学报, 2017, 30(5):1104-1108.
[16] 郑世燕, 丁伟, 陈弟军, 等. 根际土壤调控对连作烟田青枯病的控制作用[J]. 中国烟草学报, 2013, 19(1):47-52.
[17] Liu L J, Sun C L, Liu X X, et al. Effect of calcium cyanamide, ammonium bicarbonate and lime mixture, and ammonia water on survival of Ralstonia solanacearum and microbial community[J]. Scientific Reports, 2016(6):19037.
[18] 孙建, 饶月亮, 乐美旺, 等. 干旱胁迫对芝麻生长与产量性状的影响及其抗旱性综合评价[J]. 中国油料作物学报, 2010, 32(4):525-533.
[19] 李信申, 黄小梅, 魏林根, 等. 芝麻青枯病发生特点及药剂防治技术[J]. 中国油料作物学报, 2019, 41(6):932-937.
[20] Rousk J, Baath E, Brookes P C, et al. Soil bacterial and fungal communities across a pH gradient in an arable soil[J]. The ISME Journal, 2010(4):1340-1351.
[21] Li S, Liu Y, Wang J, et al. Soil acidification aggravates the occurrence of bacterial wilt in south China[J]. Fronters in Microbiology, 2017, 8:703.
[22] She S Y, Niu J J, Zhang C, et al. Significant relationship between soil bacterial community structure and incidence of bacterial wilt disease under continuous cropping system[J]. Archives of Microbiology, 2017(199):267-275.
[23] 朱昌雄, 蒋细良, 孙东园, 等. 新农用抗生素:中生菌素[J]. 精细与专用化学品, 2002(16):14-17.
[24] 蒋细良, 王劲波, 王慧敏, 等. 中生菌素对水稻悬浮细胞过氧化物酶基因转录表达的影响[J]. 中国生物防治, 2006, 22(3):207-210.
[25] 胡小梅, 徐进, 毛连刚, 等. 7种杀菌剂对姜瘟病菌Z-AQ-2菌株的抑菌活性[J]. 植物保护, 2016, 42(6):208-214.
[26] 龙厚茹, 谢德龄. 中生菌素对马铃薯青枯病菌的溶菌作用[J]. 中国生物防治, 1999, 15(2):95-96.
[27] 王梅, 尹显慧, 龙友华, 等. 几种杀菌剂对番茄青枯病菌的毒力测定及田间药效[J]. 江苏农业科学, 2015, 43(4):151-153.
[28] 周开拓, 蒋承耿, 王秋萍, 等. 中生菌素对贵州烟区烟草青枯病的毒力测定及田间防效试验[J]. 农业科技与装备, 2017(11):14-16.
[29] 朱志刚, 胡洪涛, 石丽桥, 等. 生姜青枯病防治药剂室内筛选及评价[J]. 植物保护, 2017, 43(6):202-206.
[30] 龙友华, 罗德勇. 3%中生菌素可湿性粉剂防治番茄青枯病的效果初探[J]. 中国蔬菜, 2009(6):63-66.