PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus

doi:10.16409/j.cnki.2095-039x.2024.02.027

Abstract

Abstract: Lysobacter, a genus of Gram-negative bacteria, is emerging as a novel source of biocontrol agents against phytopathogenic microorganisms. Previously, we isolated an N-oxide phenazine myxin from Lysobacter antibioticus OH13, demonstrating potent activity against bacteria, fungi, and oomycetes. Furthermore, we elucidated the biosynthetic pathway of myxin, however, there are few reports concerning its regulatory mechanisms. In this study, we identified a PAS-LuxR family transcriptional regulator encoding gene, LaPhzR, located upstream of the myxin biosynthetic gene cluster. Deletion of LaPhzR resulted in deficiency of myxin production and antagonism against Xanthomonas oryzae pv. oryzicola (Xoc) RS105. Additionally, the LaPhzR mutant exhibited impaired expression of myxin biosynthetic genes and detoxification gene LaPhzX. These findings indicate that LaPhzR positively regulates myxin biosynthesis, a crucial process for myxin production. Overexpression of LaPhzR in the wild type strain led to 1.6-fold increase in myxin production, highlighting its significance in myxin development.

Key words: Lysobacter, phenazine, myxin, transcriptional regulator, PAS-LuxR

CLC Number:

S476

ZHAO Yangyang, CHEN Junjing, XU Gaoge, CHENG Xinyi, LIU Fengquan. PAS-LuxR Family Transcription Factor Positively Regulates the Biosynthesis of Antimicrobial Phenazine Myxin in Lysobacter antibioticus[J]. Chinese Journal of Biological Control, 2024, 40(4): 866-873.

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