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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (1): 230-241.DOI: 10.16409/j.cnki.2095-039x.2022.02.003

• RESEARCH REPORTS • Previous Articles     Next Articles

Study on the Resistance Mechanism of Burkholderia sp. JP2-270 against Rice Sheath Blight

WU Lijuan, HAN Cong, WANG Huimei, WANG Lei, E Zhiguo   

  1. China National Rice Research Institute, Hangzhou 311401, China
  • Received:2021-09-10 Online:2022-02-08 Published:2022-03-01

Abstract: Sheath blight, caused by Rhizoctonia solani, is one of the three major diseases of rice. In this study, we performed gene knockout, real time quantitative PCR and differential analysis of targeted metabolites to preliminarily uncover the inhibitory mechanism of Burkholderia sp. JP2-270 against rice sheath blight. Magnaporthe oryza, Fusarium oxysporum, Fusarium graminearum and Septoria lycopersici Speg were selected as the target fungi for inhibition activity analysis, and the results showed that JP2-270 has broad spectrum inhibitory activities against these phytopathogenic fungi. Targeted gene knockout analysis indicated that pyrrolnitrin produced by strain JP2-270 was the main secondary metabolite inhibiting R. solani GD118. qRT-PCR analysis showed that the expression of gene cluster (prnA-D) responsible for the production of pyrrolnitrin was positively regulated by a LysR transcriptional regulator BysR in strain JP2-270, and the differential metabolite spectrum analysis further confirmed that BysR could positively regulate the synthesis of pyrrolnitrin. LysR type transcriptional regulatory protein BysR was reported for the first time as the regulator of pyrrolnitrin production in Burkholderia. The transcriptional regulator BysR could be used as a target of genetic engineering to improve the production of pyrrolnitrin. Thus, Burkholderia sp. JP2-270 has the potential to be developed as a biocontrol agent against plant fungal disease.

Key words: Burkholderia, rice sheath blight disease, pyrrolnitrin, transcriptional regulator BysR

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