Identification and Expression Profiling of Lipase Genes in Arma custos

doi:10.16409/j.cnki.2095-039x.2024.03.005

Abstract

Abstract: Lipase plays an important role in lipid absorption, transport, and utilization in insects. In this study, 44 lipase genes were identified in the Arma custos genome by homologous alignment. They are divided into 24 neutral lipases, 16 acid lipases, one GDSL lipase, two lipase 3 and one hormone sensitive lipase. Reverse transcription- polymerase chain reaction (RT-PCR) was used to analyze the expression characteristics of these lipase genes in different tissues of A. custos adults. The results showed that the AcusN3a, AcusN5b, AcusN18c, AcusN22, AcusA13a, AcusLipase3b, AcusGDSL1, and AcusHSL1A genes were highly expressed in the venom apparatus (salivary gland). AcusN10a, AcusN18b, AcusN20a, AcusN20b, AcusN22, AcusA7a, AcusA7b, AcusA8, AcusA9b, AcusA13a, and AcusA15 genes were highly expressed in the gut. The lipase activities in the different glands of venom apparatus and gut of A. custos were assayed. The results showed that the lipase activities in the anterior main gland, posterior main gland, and gut were about 23, 21, and 41 U/mg protein, respectively. These results lay the foundation for further study on the physiological functions of Arma custos lipase genes involved in venom and digestion.

Key words: Arma custos, venom, lipase, digestion, expression profile

CLC Number:

S476.2

WANG Yuzhi, LIU Sha, LIANG Wenkai, WANG Yuqin, ZHU Jiaying. Identification and Expression Profiling of Lipase Genes in Arma custos[J]. Chinese Journal of Biological Control, 2025, 41(1): 32-43.

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