Effect of Variable Temperature and Rapid Cold Hardening on Growth and Development of Trichopria drosophilae

doi:10.16409/j.cnki.2095-039x.2024.07.015

Abstract

Abstract: Trichopria drosophilae is an important parasitoid in pupal stage of Drosophila suzukii. After parasitizing D. suzukii, T. drosophilae develops in host pupae, so as to realize the control of D. suzukii. As a practical storage and treatment method for natural enemies, low temperature plays an important role in extending the shelf life of natural enemies. Using emergence rate and sex ratio as indexes, the effects of variable temperature and rapid cold hardening on cold storage of T. drosophilae were studied. The results of variable temperature test showed that there was no significant difference in emergence rate of T. drosophilae between treatment and control group (76.64%) after storage with T5 at 4^th instar larvae stage for 10 (72.62%) and 15 days (65.99%). Under the same temperature, the emergence rate of 4^th instar larval T. drosophilae decreased with the extension of storage time. Under the same torage time, the emergence rate of 4^th instar larval T. drosophilae was significantly higher than that under constant temperature treatment. There was no difference in sex ratio of emerging adults after the same variable temperature treatment and different storage time. The rapid cold hardening experiment showed that there was no significant difference in the emergence rate of 4^th instar larval and 1 day old pupal T. drosophilae after cold hardening. At 10 and 15 days of storage, the sex ratio of 4^th instar larval T. drosophilae with rapid cold hardening was significantly higher than that of the control and direct cold storage. At the same storage temperature, the sex ratio of 4^th instar larval and 1 day old pupal T. drosophilae emerged after rapid cold hardening did not change significantly with the extension of storage time. The results of this study can provide a reference for low temperature storage and rapid cold hardening before low temperature storage, and provide an important basis for extending the shelf life of natural enemy insects.

Key words: Drosophila suzukii, Trichopria drosophilae, cold storage, variable temperature, cold hardening

CLC Number:

S433
S477+.1

ZHANG Qinyuan, LIU Xuxiang, CHEN Jun, WU Yuhong, XU Weijie, FAN Qingwen, YANG Yongbang, CHEN Shi, JI Qinge. Effect of Variable Temperature and Rapid Cold Hardening on Growth and Development of Trichopria drosophilae[J]. Chinese Journal of Biological Control, 2025, 41(2): 251-259.

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