Effects of Different Storage Methods on the Reproductive Potential of Trichogramma dendrolimi

doi:10.16409/j.cnki.2095-039x.2025.01.020

Abstract

Abstract: This study aimed to investigate the differences in reproductive potential of Trichogramma dendrolimi after diapause induction and cold storage. The findings are expected to provide references for the mass-rearing, storage, and application of T. dendrolimi. In this research, the Trichogramma insects were either maintained a 30-day diapause or kept in a 30-day cold storage. Seven biological indicators were measured: the number of parasitized host eggs, the proportion of wasp eggs laid on the first day, the number of wasp eggs in ovary, the proportion of mature wasp eggs, longevity, oviposition duration, and duration of laying behavior. The results showed that, compared with the wasps maintained at normal temperature, diapause and cold storage significantly increased the number of eggs in ovary and lifetime eggs, and decreased the proportion of eggs laid on the first day. Diapause significantly increased the proportion of mature eggs and longevity in T. dendrolimi, while refrigeration led to a decline in both indicators. In addition, diapause treatment significantly shortened the time required for laying behavior, while cold storage increased the time required. However, no significant differences in oviposition duration were observed between the three treatments. Compared with cold storage wasps, diapause T. dendrolimi were superior in all the indexes investigated in this study. When the storage duration was 30 days, diapause obviously improved the overall quality of T. dendrolimi, while cold storage had a certain degree of negative impact.

Key words: diapause, cold storage, fecundity, oviposition behavior

CLC Number:

S476.3

LI Hui, ZHANG Qianyu, ZHENG Meixin, SONG Xiaoyang, GUO Jiaqing, ZHANG Xue, ZHANG Junjie, SHI Hongwei. Effects of Different Storage Methods on the Reproductive Potential of Trichogramma dendrolimi[J]. Chinese Journal of Biological Control, 2025, 41(6): 1293-1299.

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