Preparation and Performance Evaluation of Metarhizium pingshaense Alginate Granules Based on Sodium Alginate

doi:10.16409/j.cnki.2095-039x.2025.02.029

Abstract

Abstract: Metarhizium spp. are important biocontrol fungal resources for controlling underground pests. Due to their susceptibility to environmental conditions, they are not conducive to commodity storage and forest applications. In order to improve the survival activity of M. pingshaense, this study investigated the alginate granules form of M. pingshaense strain WP08. The alginate granules were prepared by titration granulation method, and the effects of spore powder concentration, particle diameter, filling carrier, and nutrient activator on the granulation characteristics and spore production of alginate granules were determined. The UV resistance and storage effect of alginate granules were also tested. The results showed that the alginate particles of the WP08 strain of M. pingshaense were spherical in shape and exhibited excellent mechanical compressive strength; the optimum conditions were 0.75% spore powder concentration, 8% 1250 mesh attapulgite as carrier, 0.5% rice flour as nutrient activator, and 6 mm particle diameter. After 30 minutes of ultraviolet UV irradiation, the spore production yield using a carrier of 1250-mesh attapulgite increased by 2.89-fold compared to the control group. Following 5 months of storage, the spore yield of alginate granules increased by 1.31-fold, demonstrating superior storage performance relative to the WP08 spore powder and the rice based granules control groups. These results indicate that the formulation exhibits enhanced UV resistance and improved storage stability. The alginate granules prepared by the WP08 strain of M. pingshaense in this study have broad prospects for underground pest control.

Key words: Metarhizium pingshaense, sodium alginate, nutrient booster, crosslinking reaction, alginate granules

CLC Number:

S476

LIU Yaohui, SHEN Zhilai, LIANG Shuang, LI Zijian, SHU Jinping, ZHANG Yabo, ZHAI Fengyan. Preparation and Performance Evaluation of Metarhizium pingshaense Alginate Granules Based on Sodium Alginate[J]. Chinese Journal of Biological Control, 2025, 41(3): 617-626.

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