Alginate Granule Formulation Promotes Recovery of Metarhizium anisopliae Population

doi:10.16409/j.cnki.2095-039x.2016.06.013

Abstract

Abstract: To ensure viability of Metarhizium anisopliae and promotion of their proliferation in field, the key factors of alginate granulation with the fungus, such as gel density, carriers, active components and out membrane were investigated. The optimal granulating conditions of cross linking reaction by sodium alginate and calcium were 2% sodium alginate and 0.2-0.3 mol/L calcium chloride. Padding with 4%-10% attapulgite inside the gel granules added to fungal survival and recovery. Adding 0.5% corn flour or dextrin as pro-live agent was conducive to the fungus restoration and increased conidiation by 60%. The out membrane around granule composed of 1.5% sodium alginate and 3.5% polyvinyl alcohol had a role to withstand the attacks from environmental microorganisms.

Key words: Metarhizium anisopliae, formulation, sodium alginate, granules, population recovery

CLC Number:

S476.12

NONG Xiangqun, WANG Guangjun, ZHANG Xiongpeng, LIU Xun, ZHANG Zehua. Alginate Granule Formulation Promotes Recovery of Metarhizium anisopliae Population[J]. journal1, 2016, 32(6): 762-766.

References

[1] Groden E, Lockwood J L. Effects of soil fungistasis on Beauveria bassiana and its relationship to disease incidence in the Colorado potato beetle, Leptinotarsa decemlineata, in Michigan and Rhode Island soils[J]. Journal of Invertebrate Pathology, 1991, 57(1):7-16.
[2] Strasser H, Abendstein D, Stuppner H, et al. Monitoring the distribution of secondary metabolites produced by the entomogenous fungus Beauveria brongniartii with particular reference to oosporein[J]. Mycological Research, 2000, 104(10):1227-1233.
[3] Cagan L, Svercel M. The influence of ultraviolet light on pathogenicity of entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin to the European corn borer, Ostrinia nubilalis Hbn.(Lepidoptera:Crambidae)[J]. Journal of Central European Agriculture, 2001, 2(3/4):227-234.
[4] 农向群, 张泽华, 金正俊. 昆虫病原真菌抗紫外保护剂的筛选和评价[J]. 植物保护学报, 2005, 32(4):402-406.
[5] 郁建平, 郭刚军, 肖云鹏, 等. 海藻酸钠固定化多酚氧化酶及红棓酚的合成研究[J]. 有机化学, 2003, 23(1):57-61.
[6] 张富新, 张媛媛, 党亚丽, 等. 海藻酸钠固定化中性蛋白酶的研究[J]. 西北农林科技大学学报(自然科学版), 2005, 33(11):89-93.
[7] 李宝红, 陈建海, 赵志玲. 缓释片剂中几种药剂新辅料的应用[J]. 解放军药学学报, 2001, 17(4):207-210.
[8] 李辉华, 谭洪新, 罗国芝, 等. 固定化微生物技术及其在闭合循环养殖系统水处理中的应用[J]. 水产科技情报, 2001, 28(2):51-54.
[9] 李超敏, 韩梅, 张良, 等. 根瘤菌-光合细菌复合型生物肥料固定化包埋的初步研究[J]. 山东农业科学, 2006(3):51-53.
[10] Pereira R M, Roberts D W. Alginate and cornstarch mycelial formulations of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae[J]. Journal of Economic Entomology, 1991, 84(6):1657-1661.
[11] Bextine B R, Thorvilson H G. Field applications of bait-formulated Beauveria bassiana alginate pellets for biological control of the red imported fire ant (Hymenoptera:Formicidae)[J]. Environmental Entomology, 2002, 31(4):746-752.
[12] 农业部农药鉴定所. 真菌农药母药产品标准编写规范[P]. 中华人民共和国国家标准GB/T 21459.1-2008.
[13] 章小忠, 梅乐和, 姚善泾. 纤维素硫酸钠的制取反应过程研究及由其制备的微胶囊性能[J]. 化学反应工程与工艺, 2003, 19(3):254-259.
[14] Nong X Q, Liu X, Wang Q L, et al. Phoxim and manure enhanced the population rebound and preservation of Metarhizium anisopliae applied in soil[J]. Biocontrol Science and Technology, 2015, 25(1):84-96.
[15] 蒋宇红, 黄霞, 俞毓馨. 几种固定化细胞载体的比较[J]. 环境科学, 1994, 14(2):11-15.
[16] 杨海波, 于媛, 张欣华, 等. 小球藻固定化培养的初步研究[J]. 水产科学, 2001(5):4-7.