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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (3): 396-404.DOI: 10.16409/j.cnki.2095-039x.2020.03.016

• RESEARCH REPORTS • Previous Articles     Next Articles

Bio-Fertilizer Optimization and Its Control Effect on Banana Fusarium Wilt

DU Chanjuan1, YANG Di1, PAN Lianfu1, YE Yunfeng2, ZHANG Jin1, FU Gang1   

  1. 1. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Institute of Plant Protection Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
    2. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2019-10-11 Online:2020-06-08 Published:2020-06-12

Abstract: In order to improve the living conditions of antagonistic fungus in the fertilizer and improve control effect of the bio-fertilizer on banana Fusarium wilt, a variety of factors related to the growth of antagonistic strain were optimized based on the GFP-labeled strain Thichoderma harzianum GFP-gz-2. The effects of the bio-fertilizer on disease control and growth promotion were evaluated before and after optimization, respectively. The results showed that glutinous rice flour was the best one among the 7 carbon sources for the growth of strain GFP-gz-2. The colony diameter and sporulation of strain GFP-gz-2 reached the maximum when the content of glutinous rice flour was 35 g/L. Ca2+ was the best one in the 6 metal ions for the growth of strain GFP-gz-2. The results of multi-factor orthogonal optimization showed that the best conditions for the growth of strain GFP-gz-2 in the bio-fertilizer were listed as follows:C/N ratio of organic fertilizer was 30:1, concentration of Ca2+was 5%, and the inoculation amount of the strain was 2.0×106 spore/g. Under these conditions, the colonization amount of strain GFP-gz-2 in the bio-fertilizer was 1.033×108 spore/g. The control effect of optimized bio-fertilizer on banana Fusarium wilt reached to 84.75%, 15.26% higher than that before optimization. The optimized bio-fertilizer increased the plant height, stem girth, fresh weight and dry weight of banana significantly, which were 23.35%, 22.82%, 68.78% and 50.17% higher than that before optimization, respectively.

Key words: banana Fusarium wilt, Trichoderma harzianum, fungus fertilizer, formulation optimization

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